Tagmentation Buffer (1x), 1mL

Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode Tagmentase (Tn5 transposase) on low volume samples (for example DNA or chromatin immobilized on magnetic beads), because it does not require any dilution. This is the reagent used in Diagenode ChIPmentation solutions.

ChIPmentation experiments:

• After Immunoprecipitation, the magnetic beads binding the antibody/chromatin complex are washed and can then be incubated with the following mix for 1 reaction :

*  The quantity of chromatin per reaction will depend on the ChIPmentation experimental design. Successful tagmentation with the proposed protocol has been performed on chromatin from 5,000 to 4,000,000 cells per reaction.

• The reaction is then incubated 10 minutes at 37°C.
• The tagmentation reaction can then be stopped on ice with addition of a buffer containing SDS (0.1%).