Thermo Scientific™ Oxoid™ Nitrocefin Solution, 2 x 5 Vials

Thermo Scientific™ Oxoid™ Nitrocefin Solution is a chromogenic reagent designed for the rapid qualitative detection of β-lactamase production in pathogenic microorganisms. β-lactamase enzymes are responsible for resistance to penicillins and many cephalosporins through hydrolysis of the β-lactam ring. Rapid identification of β-lactamase-producing organisms supports accurate antimicrobial therapy decisions and resistance monitoring.

This product is supplied in a lyophilized format with a dedicated rehydrating fluid to ensure reagent stability and optimal performance.

Principle of the Method

Nitrocefin is a chromogenic cephalosporin substrate.

When β-lactamase is present:

• The enzyme hydrolyzes the β-lactam ring of nitrocefin.
• The substrate undergoes a visible color change from pale yellow to pink/red.
• The color change indicates β-lactamase production.

Organisms that do not produce β-lactamase will not cause a color change within the specified test period.

The chromogenic method provides a sensitive and rapid alternative to iodometric and acidimetric β-lactamase detection techniques.

Product Configuration

Catalog Number: SR0112C

Packaging: 10 Vial Set per Pack (2 × 5 Vials)

Each pack contains:

• 5 vials lyophilized nitrocefin (1 mg per vial)
• 5 vials rehydrating fluid (1.9 mL per vial)

Upon reconstitution, the solution yields a concentration of 500 µg/mL.

Key Features

• Lyophilized format for enhanced stability
• Rapid chromogenic color reaction
• High sensitivity for β-lactamase detection
• Suitable for a wide range of pathogenic bacteria
• Simple preparation and use

Applications

• Clinical microbiology laboratories
• Detection of penicillinase and cephalosporinase production
• Rapid screening for antimicrobial resistance
• Testing of Gram-positive and Gram-negative pathogens
• Support of antimicrobial susceptibility workflows

Commonly tested organisms include:

• Neisseria gonorrhoeae
• Haemophilus influenzae
• Moraxella catarrhalis
• Staphylococcus spp.
• Other β-lactamase-producing pathogens

Storage and Handling

Store according to manufacturer recommendations.

Reconstituted solution should be handled as per laboratory protocol.

For professional laboratory use only.

Specifications

Thermo Scientific Oxoid Nitrocefin Solution provides a reliable and sensitive chromogenic method for detecting β-lactamase production in pathogenic microorganisms. Supplied in a stable lyophilized format, it supports rapid antimicrobial resistance screening in clinical microbiology laboratories and contributes to informed therapeutic decision-making.