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Specifications:
| Application | qPCR, Reverse Transcription | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | SolisBiodyne | ||
| Product Grade | Molecular Biology | ||
The SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use reagent formulated for fast, highly sensitive, and reproducible probe-based qPCR assays. Optimized for use with dual-labeled hydrolysis probes (e.g., TaqMan®), this mix supports the detection and quantification of up to five targets simultaneously, making it an excellent choice for multiplex qPCR applications.
By combining the novel in silico-designed SolisFAST® DNA Polymerase with a highly optimized buffer system, this mix ensures robust amplification, accurate detection, and reliable quantification even in challenging samples. With enhanced stability, it enables ice-free shipping and reaction setup, simplifying logistics without compromising performance.
Key Features
- Ultra-Fast Performance: Delivers qPCR results up to 2x faster, in as little as 30 minutes.
- High Multiplexing Capability: Supports up to 5-plex assays for simultaneous target detection.
- Reliable Detection: Consistent performance across low- and high-copy targets.
- Room Temperature Stability: Stable for 6 months at 15–25 °C, reducing cold-chain requirements.
- Robust Design: Tolerant to common PCR inhibitors for challenging sample matrices.
- Hot-Start Technology: 3-minute initial incubation at 95 °C prevents non-specific amplification.
- Flexible Compatibility: Available in No ROX, ROX, and Purple reference dye formats for compatibility with a wide range of qPCR cyclers.
Applications
- DNA/LNA hydrolysis probe-based assays
- Detection and quantification of DNA and cDNA targets
- Gene expression profiling
- Microbial detection
- Viral load determination
Technical Specifications
| Property | Details |
|---|---|
| Concentration | 5x |
| Hot-start | Yes (3 min at 95 °C) |
| Cycling Speed | Fast and ultra-fast |
| Detection Type | Probe-based (e.g., TaqMan® probes) |
| Multiplex Capability | Up to 5-plex |
| Reference Dye Options | No ROX, ROX, Purple |
| Compatible Instruments | qPCR cyclers supporting respective dye references |
| MgCl₂ (final conc.) | 3 mM at 1x |
| Stability at Room Temp. | 6 months (15–25 °C) |
| Storage (long-term) | –20 °C |
| Storage (short-term) | +4 °C (up to 1 year) |
| Freeze-Thaw Stability | 30 cycles |
| Shipping | Ambient temperature |
Mix Components
- SolisFAST® DNA Polymerase: Engineered Taq analogue with superior stability, rapid hot-start, and faster extension rates.
- qPCR Buffer: Contains 15 mM MgCl₂ (3 mM final at 1x), dNTPs (dATP, dCTP, dGTP, dTTP), and proprietary additives for maximum efficiency.
- Reference Dye: Available in formats with No ROX, ROX, or Purple dye for instrument-specific normalization.
Ordering Information
| Product | Catalog Number | Pack Size | Reactions |
|---|---|---|---|
| SolisFAST® Probe qPCR Mix (No ROX) | 28-01-0000S | 0.2 mL | 50 rxn |
| 28-01-00001 | 1 mL | 250 rxn | |
| 28-01-00001 | 5 mL (5 × 1 mL) | 1250 rxn | |
| 28-01-00001 | 20 mL (20 × 1 mL) | 5000 rxn | |
| 28-01-00001 | 50 mL (50 × 1 mL) | 12,500 rxn | |
| 28-01-00001-5 | 5 mL (5-pack: 5 × 1 mL) | 1250 rxn | |
| 28-01-00001-5 | 20 mL (4 × 5-pack) | 5000 rxn | |
| 28-01-00020 | 20 mL | 5000 rxn | |
| SolisFAST® Probe qPCR Mix (ROX) | 28-02-0000S | 0.2 mL | 50 rxn |
| 28-02-00001 | 1 mL | 250 rxn | |
| 28-02-00001 | 5 mL (5 × 1 mL) | 1250 rxn | |
| 28-02-00001 | 20 mL (20 × 1 mL) | 5000 rxn | |
| 28-02-00001 | 50 mL (50 × 1 mL) | 12,500 rxn | |
| 28-02-00001-5 | 5 mL (5-pack: 5 × 1 mL) | 1250 rxn | |
| 28-02-00001-5 | 20 mL (4 × 5-pack) | 5000 rxn | |
| 28-02-00020 | 20 mL | 5000 rxn | |
| SolisFAST® Probe qPCR Mix (Purple) | 28-03-0000S | 0.2 mL | 50 rxn |
| 28-03-00001 | 1 mL | 250 rxn | |
| 28-03-00001 | 5 mL (5 × 1 mL) | 1250 rxn | |
| 28-03-00001 | 20 mL (20 × 1 mL) | 5000 rxn | |
| 28-03-00001 | 50 mL (50 × 1 mL) | 12,500 rxn | |
| 28-03-00001-5 | 5 mL (5-pack: 5 × 1 mL) | 1250 rxn | |
| 28-03-00001-5 | 20 mL (4 × 5-pack) | 5000 rxn | |
| 28-03-00020 | 20 mL | 5000 rxn |
The SolisFAST® Probe qPCR Mix is a powerful, versatile solution for researchers requiring fast, accurate, and multiplex probe-based qPCR. With flexible formats (No ROX, ROX, Purple), extended room temperature stability, and tolerance to inhibitors, it is well-suited for high-throughput laboratories, gene expression studies, microbial and viral detection, and other advanced molecular biology applications.
- Choose Mix: ROX No ROX Purple
- Pack Size: 1 x 1 mL, sufficient for 250 Rxns 5 x 1 mL, sufficient for 1250 Rxns 1 x 20 mL, sufficient for 5000 Rxns 0.2 ml, 50 x 20 μl rxn, free sample 1 x 5 mL, Sufficient for 1250 Rxns 4 x 5 mL, Sufficient for 5000 Rxns 1 x 50 mL, Sufficient for 12,500 Rxns