SolisFAST® Master Mix

The SolisFAST® Master Mix is a 5× concentrated, ready-to-use reagent designed for extremely fast endpoint PCR assays. Powered by the in silico–engineered SolisFAST® DNA Polymerase and an optimized buffer system, it delivers robust, reliable amplification in both singleplex and multiplex PCR reactions. Results can be obtained in as little as 20 minutes, making it one of the fastest PCR solutions available for endpoint detection.

This versatile mix supports amplification of up to 5 kb from low-complexity templates (e.g., plasmid or lambda DNA) and 3 kb from complex genomic DNA, with robust performance suitable for cloning, genotyping, and sequencing workflows.

Key Features & Benefits

• Ultra-Fast Performance – Endpoint PCR results in just 20 minutes.
• Single & Multiplex Compatibility – Reliable amplification for 1–18 targets.
• High Stability – Stable for 2 months at room temperature and up to 1 year at +4°C.
• Freeze–Thaw Resistant – Withstands up to 30 cycles without performance loss.
• Hot-Start Technology – Prevents nonspecific amplification, 2 min activation at 98 °C.
• Sanger Sequencing Compatible – Amplified DNA suitable for downstream sequencing.
• Environmentally Friendly Shipping – Stable at room temperature, eliminating the need for ice.

Applications

• Endpoint PCR assays
• Genotyping and screening workflows
• Cloning (T/A cloning compatible)
• Sanger sequencing sample preparation
• Multiplex PCR (up to 18 targets depending on length)

Mix Properties

• Concentration: 5×
• Amplicon Size: Up to 5 kb (low-complexity DNA); up to 3 kb (gDNA)
• Targets: 1–18
• Overhangs: T/A cloning compatible
• Hot-Start: Yes (2 min at 98 °C)
• Ready-to-Load: No
• Buffer Components:
  • 17.5 mM MgCl₂ (final 3.5 mM in 1× PCR)
  • dNTPs (dATP, dCTP, dGTP, dTTP)
  • Proprietary additives for efficiency

Storage & Stability

• Routine Storage: –20 °C until expiry date
• Refrigerated Storage: +4 °C up to 1 year
• Room Temperature Stability: 2 months (15–25 °C)
• Freeze–Thaw Stability: Up to 30 cycles
• Shipping: Room temperature

Ordering Information

The SolisFAST® Master Mix is a high-speed, high-stability PCR solution designed for robust endpoint detection in both simple and complex assays. With fast turnaround times, multiplexing capacity, and compatibility with downstream sequencing, it is ideal for research laboratories requiring reliable and efficient PCR performance.