Human anti-Thyroxine Binding Globulin (anti-TBG) ELISA Kit

The Human Anti-Thyroxine Binding Globulin (anti-TBG) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of anti-TBG antibody levels in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research into autoimmune thyroid disorders, thyroid hormone transport, and related immune responses.

Purpose

• Quantification of Anti-TBG Levels:
  • Detect and measure anti-TBG antibody concentrations in human biological samples for research into thyroid autoimmunity and hormonal transport.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to ensure accurate and reliable quantification of anti-TBG. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antigen specific to anti-TBG.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where anti-TBG antibodies in the sample bind to the immobilized antigen.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antigen specific to TBG is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing anti-TBG.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • Anti-TBG concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of anti-TBG with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of anti-TBG concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• Anti-TBG standards for standard curve generation.
• HRP-conjugated TBG detection antigen.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Autoimmune Thyroid Research:
  • Study anti-TBG as a biomarker for autoimmune thyroid disorders and thyroid hormone transport.
• Endocrine Studies:
  • Investigate the relationship between anti-TBG levels and thyroid function.
• Clinical Diagnostics Research:
  • Assess anti-TBG as a marker for thyroid autoimmunity and related immune responses.
• Therapeutic Development:
  • Monitor the effectiveness of treatments targeting autoimmune thyroid disorders.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of anti-TBG.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated TBG antigen.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate anti-TBG concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Anti-Thyroxine Binding Globulin (anti-TBG) ELISA Kit is a vital tool for researchers studying autoimmune thyroid disorders, thyroid hormone transport, and related immune responses. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.