Human Des-Gamma-Carboxy-Prothrombin (Dcp) Elisa Kit - 96 Tests

Human Des-Gamma-Carboxy Prothrombin (DCP) ELISA Kit

The Human Des-Gamma-Carboxy Prothrombin (DCP) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of DCP levels in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research on hepatocellular carcinoma (HCC), liver function, and related disorders.

Purpose

• Quantification of DCP Levels:
  • Measures DCP concentrations as a biomarker for liver diseases, particularly hepatocellular carcinoma (HCC).
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the specific and accurate measurement of DCP. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to DCP.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing DCP in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to DCP is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with DCP.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • DCP concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of DCP with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of DCP concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• DCP standards for standard curve generation.
• HRP-conjugated DCP detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Liver Disease Research:
  • Investigate DCP as a biomarker for liver function and hepatocellular carcinoma (HCC).
• Cancer Research:
  • Study DCP as a diagnostic and prognostic marker for HCC.
• Clinical Diagnostics Research:
  • Assess DCP levels in various liver-related diseases.
• Therapeutic Monitoring:
  • Monitor the effects of therapeutic interventions targeting HCC.
• Drug Development:
  • Evaluate potential drugs affecting DCP-related pathways.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of DCP.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated DCP antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate DCP concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Des-Gamma-Carboxy Prothrombin (DCP) ELISA Kit is a vital tool for detecting and quantifying DCP levels in biological samples. Its high sensitivity, specificity, and user-friendly design make it indispensable for liver disease research, particularly in hepatocellular carcinoma (HCC).