Human intestinal fatty acid binding protein, iFABP ELISA Kit

The Human Intestinal Fatty Acid Binding Protein (iFABP) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of iFABP levels in human serum, plasma, culture media, urine, and other biological fluids. This kit is essential for research into intestinal permeability, gut health, and related disorders.

Purpose

• Quantification of iFABP Levels:
  • Detect and measure iFABP concentrations in human biological samples to study intestinal barrier function, gut health, and tissue injury.
• Sample Compatibility:
  • Suitable for serum, plasma, urine, culture media, and other biological fluids.

Sample Preparation

1. Serum Preparation

1. Collect whole blood and allow it to clot at room temperature for 10–20 minutes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes to separate the serum.
3. Re-centrifuge if precipitates appear during storage.

2. Plasma Preparation

1. Collect whole blood into tubes containing anticoagulants (e.g., EDTA or citrate).
2. Incubate at room temperature for 10–20 minutes.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes to obtain plasma.
4. Re-centrifuge if necessary.

3. Urine Samples

1. Collect urine in aseptic tubes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes and carefully collect the supernatant.
3. Re-centrifuge if precipitates form during storage.

4. Cell Samples

1. For cell secretions, collect culture supernatants in aseptic tubes and centrifuge at 2,000–3,000 rpm for 20 minutes.
2. For intracellular components:
   • Dilute cells in PBS (pH 7.2–7.4) at 1×10⁷/mL.
   • Disrupt cells via repeated freeze-thaw cycles.
   • Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

5. Tissue Samples

1. Cut and weigh tissue samples, then freeze in liquid nitrogen and store at -80°C.
2. Homogenize samples in PBS (pH 7.4) at 4°C.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

Important Notes

1. Perform ELISA assays as soon as possible after sample collection.
2. Store samples at -20°C if immediate testing is not feasible.
3. Avoid repeated freeze-thaw cycles to preserve sample integrity.
4. Do not use samples containing sodium azide (NaN₃) as it inhibits HRP activity.

Applications

• Intestinal Health Research:
  • Study iFABP as a biomarker for gut barrier integrity and intestinal injury.
• Gastrointestinal Disorder Studies:
  • Investigate iFABP levels in conditions like inflammatory bowel disease (IBD) and celiac disease.
• Clinical Diagnostics Research:
  • Assess iFABP as a biomarker for intestinal permeability and systemic inflammation.
• Tissue Damage Research:
  • Monitor iFABP in diseases associated with ischemia or tissue injury.
• Therapeutic Development:
  • Evaluate treatments targeting gut health and intestinal barrier restoration.

Advantages

1. High Sensitivity and Specificity:
   • Detects low levels of iFABP with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, cell culture supernatants, and tissue homogenates.
3. Ease of Use:
   • Pre-coated plates and ready-to-use reagents simplify assay execution.
4. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Intestinal Fatty Acid Binding Protein (iFABP) ELISA Kit is a critical tool for researchers studying intestinal health, gut barrier function, and tissue injury. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.