Mouse Acetylcholinesterase (AChE)ELISA Kit

The Mouse Acetylcholinesterase (AChE) ELISA Kit is a reliable and precise tool for quantifying AChE levels in various mouse biological samples, including serum, plasma, culture media, and other biological fluids. This kit employs a Sandwich-ELISA methodology to provide highly specific and sensitive results, enabling researchers to accurately assess AChE concentration for research purposes.

Purpose:

This kit is designed for:

• Detecting and quantifying Acetylcholinesterase (AChE) in mouse samples.
• Supporting research in neurobiology, toxicology, pharmacology, and related fields where AChE activity and levels are critical biomarkers.

Principle of the Assay:

The assay is based on the Sandwich-ELISA technique:

1. Antibody Coating:
   • A microplate pre-coated with an antibody specific to AChE captures the target protein from the sample.
2. Binding Phase:
   • Standards or samples are added to the wells, where AChE binds to the pre-coated antibody.
   • After incubation, an HRP-conjugated antibody specific to AChE is added to form a "sandwich" complex.
3. Washing Steps:
   • Unbound substances are washed away, leaving only the AChE-antibody complexes in the wells.
4. Signal Development:
   • TMB substrate is added, producing a blue color in the presence of HRP.
   • Upon addition of the stop solution, the color changes to yellow, which is measured spectrophotometrically at 450 nm.
5. Quantification:
   • The optical density (OD) at 450 nm is directly proportional to the concentration of AChE in the sample.
   • A standard curve is used to calculate the exact AChE concentration.

Kit Components:

• Pre-Coated Microelisa Strip Plate: Pre-coated with an anti-AChE antibody for capturing the target protein.
• AChE Standards: For generating a standard curve to quantify sample concentrations.
• HRP-Conjugated Antibody: Specific to AChE for detection.
• Sample Diluent: Ensures optimal sample conditions.
• Wash Buffer: For removing unbound components.
• TMB Substrate Solution: For colorimetric detection.
• Stop Solution: To halt the enzymatic reaction and stabilize the color.
• Instruction Manual: Detailed protocol for assay execution.

Applications:

The Mouse AChE ELISA Kit is ideal for:

• Neuroscience Research: Measuring AChE levels to study cholinergic signaling and neurodegenerative diseases.
• Toxicology Studies: Assessing the impact of toxic substances or pesticides on AChE activity.
• Pharmacological Research: Evaluating the effects of drugs targeting cholinergic pathways.
• Enzyme Activity Monitoring: Investigating AChE-related physiological or pathological processes.

Key Features and Benefits:

1. High Sensitivity and Specificity:
   • Ensures precise detection of AChE with minimal cross-reactivity.
2. Quantitative Results:
   • Provides accurate concentration values by comparing sample OD to a standard curve.
3. Versatile Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
4. Ease of Use:
   • Pre-coated plates and ready-to-use reagents simplify the workflow.
5. Rapid and Reliable:
   • Obtain results in a short assay time with clear and reproducible outputs.

Performance Characteristics:

• Detection Method: Colorimetric (450 nm OD measurement).
• Dynamic Range: Dependent on standards provided in the kit.
• Sample Volume Required: Typically optimized for small volumes.
• Sensitivity: High sensitivity to detect low levels of AChE.

Storage Conditions:

• Store all components as per manufacturer instructions, typically at 2-8°C for optimal stability.

Recommended Workflow:

1. Sample Preparation: Collect and prepare mouse biological samples (serum, plasma, or culture media). Dilute as required.
2. Plate Setup: Add standards and samples to the pre-coated wells.
3. Incubation: Allow sufficient time for binding and sandwich formation.
4. Washing: Remove unbound components with wash buffer.
5. Signal Detection: Add TMB substrate, incubate, and stop the reaction.
6. Data Analysis: Measure OD at 450 nm and calculate AChE concentrations using the standard curve.

Why Use This Kit?

• Comprehensive Research Tool: Aids in exploring cholinergic mechanisms in health and disease.
• Validated Methodology: Offers high reliability and reproducibility for experimental consistency.
• Wide Applications: Suitable for multiple research domains, including neurobiology, toxicology, and pharmacology.

For researchers investigating acetylcholinesterase levels and related biological pathways, this ELISA kit is an essential resource for accurate and efficient data acquisition.