Human Immunoglobulin M, Igm Elisa Kit, 96T

The Human Immunoglobulin M (IgM) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of IgM levels in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research into immune responses, antibody production, and immunological disorders.

Purpose

• Quantification of IgM Levels:
  • Detect and measure IgM concentrations in human biological samples for research into immunity and disease.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique for the specific and accurate quantification of IgM. Below is the process:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to IgM.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where IgM in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to IgM is added, forming a sandwich complex with the captured IgM.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing IgM.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • IgM concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of IgM with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of IgM concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a straightforward workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• IgM standards for standard curve generation.
• HRP-conjugated IgM detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Immunology Research:
  • Study IgM as a key player in humoral immunity and early immune responses.
• Infection and Vaccine Research:
  • Investigate antibody responses during acute infections and vaccination.
• Immune Disorder Studies:
  • Explore IgM levels in autoimmune diseases and immunodeficiencies.
• Therapeutic Development:
  • Monitor IgM as a biomarker in therapeutic interventions and drug efficacy.
• Clinical Diagnostics Research:
  • Support diagnostics for diseases associated with abnormal IgM levels.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of IgM.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated IgM antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate IgM concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Immunoglobulin M (IgM) ELISA Kit is a valuable tool for researchers studying immune responses, antibody production, and related immunological processes. Its precision, ease of use, and broad compatibility make it indispensable for various clinical and experimental applications.