Human Epstein-Barr virus IgG (EBv-IgG) ELISA Kit

Human Epstein-Barr Virus IgG (EBV-IgG) ELISA Kit

The Human Epstein-Barr Virus IgG (EBV-IgG) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of EBV-IgG antibodies in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research on EBV infections, immune responses, and related conditions.

Purpose

• Quantification of EBV-IgG Levels:
  • Measures EBV-IgG antibody concentrations to monitor immune response to EBV infection.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

This kit employs a Sandwich-ELISA technique for the accurate and specific detection of EBV-IgG. Below is the detailed workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an EBV-IgG-specific antigen.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing EBV-IgG antibodies in the sample to bind to the immobilized antigen.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated EBV antigen is added, forming a sandwich complex with the captured antibodies.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with EBV-IgG.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • EBV-IgG concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of EBV-IgG with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of EBV-IgG concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• EBV-IgG standards for standard curve generation.
• HRP-conjugated EBV antigen detection reagent.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Infectious Disease Research:
  • Study the immune response to Epstein-Barr Virus infections.
• Serological Studies:
  • Assess past or current EBV infections through IgG detection.
• Epidemiological Studies:
  • Investigate the prevalence of EBV in populations.
• Clinical Diagnostics Research:
  • Support diagnostics for diseases associated with EBV, such as infectious mononucleosis or EBV-related cancers.
• Vaccine Development:
  • Evaluate the immune response to potential EBV vaccines.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of EBV-IgG.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated EBV antigen.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate EBV-IgG concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Epstein-Barr Virus IgG (EBV-IgG) ELISA Kit is a valuable tool for the detection and quantification of EBV-IgG in biological samples. Its high sensitivity, specificity, and user-friendly design make it indispensable for research in infectious diseases, immune response, and EBV-associated conditions.