Human 17-Hydroxyprogesterone ,17-OHP ELISA Kit

The Human 17-Hydroxyprogesterone (17-OHP) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of 17-OHP levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into adrenal gland function, congenital adrenal hyperplasia (CAH), and related endocrine disorders.

Purpose

• Quantification of 17-OHP Levels:
  • Detect and measure 17-OHP concentrations in biological samples to study adrenal and reproductive health.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique for the accurate and specific quantification of 17-OHP. Below is the process:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to 17-OHP.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where 17-OHP in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to 17-OHP is added, forming a sandwich complex with the captured 17-OHP.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing 17-OHP.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • 17-OHP concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of 17-OHP with minimal cross-reactivity.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of 17-OHP concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• 17-OHP standards for standard curve generation.
• HRP-conjugated 17-OHP detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Endocrine Research:
  • Study 17-OHP levels in adrenal gland function and hormonal regulation.
• Congenital Adrenal Hyperplasia (CAH) Studies:
  • Monitor 17-OHP as a biomarker for CAH diagnosis and treatment monitoring.
• Reproductive Health Research:
  • Investigate 17-OHP in ovulation, menstrual cycles, and fertility.
• Therapeutic Development:
  • Assess the efficacy of treatments targeting adrenal and hormonal disorders.
• Clinical Diagnostics Research:
  • Support diagnostics for endocrine disorders involving abnormal 17-OHP levels.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of 17-OHP.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated 17-OHP antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate 17-OHP concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human 17-Hydroxyprogesterone (17-OHP) ELISA Kit is an indispensable tool for researchers studying adrenal and reproductive health. Its high sensitivity, specificity, and user-friendly design make it invaluable for clinical and experimental applications in endocrinology and hormone research.