Sigma-Aldrich Sodium chloride solution 5 M

The Sigma-Aldrich Sodium Chloride (NaCl) Solution, 5 M is a high-purity, sterile-filtered solution designed for research and further manufacturing use. It is commonly used as a biological buffer component, reagent for biochemical assays, and a critical element in cell culture, protein purification, and molecular biology protocols.

This solution contains 292.2 g/L sodium chloride and is sterile, making it suitable for sensitive applications requiring contamination-free conditions.

Key Features & Benefits

1. Ready-to-Use & Sterile

• Pre-made 5 M NaCl solution, eliminating the need for manual preparation.
• Sterile-filtered, ensuring contamination-free use in cell culture and molecular biology experiments.
• Suitable for buffer preparation, protein purification, and biochemical assays.

2. High Purity & Research-Grade

• Quality Level: 300, indicating high purity suitable for research applications.
• Free from contaminants that may interfere with biological assays.
• Used for buffer formulations, enzyme assays, and nucleic acid isolation.

3. Essential Reagent for Biological & Chemical Research

• Maintains osmotic balance in biological samples.
• Key component in buffer preparation, including PBS (phosphate-buffered saline) and TBS (Tris-buffered saline).
• Used in protein crystallization, molecular biology, and electrophoresis.

Specifications

Applications

1. Molecular Biology & Biochemistry

• Used in nucleic acid isolation to stabilize DNA and RNA.
• Essential for protein crystallization and electrophoresis buffer preparation.
• Commonly included in Western blotting and ELISA wash buffers.

2. Cell Culture & Buffer Preparation

• Used in PBS (phosphate-buffered saline) and TBS (Tris-buffered saline) preparation.
• Helps maintain osmotic balance in cell culture media.
• Ensures optimal conditions for enzyme activity in assays.

3. Enzymatic & Biochemical Assays

• Component of binding buffers for protein-protein and protein-ligand interactions.
• Used in enzyme kinetic studies, including glutamate carboxypeptidase II (NAALADase) assays.
• Helps regulate ionic strength in various biochemical reactions.

4. Industrial & Pharmaceutical Applications

• Suitable for further manufacturing processes requiring high-purity sodium chloride solutions.
• Used in diagnostic reagent formulations.

Pack Sizes & Catalog Numbers

Storage & Handling

• Store at ambient temperature.
• Avoid contamination by using sterile pipettes and handling under aseptic conditions.
• Do not freeze, as it may alter concentration or introduce precipitation.

Summary

The Sigma-Aldrich Sodium Chloride Solution (5 M, Sterile-Filtered) is a high-purity, research-grade reagent essential for molecular biology, cell culture, and biochemical assays. Its pre-made formulation eliminates preparation errors, while its sterile nature ensures contamination-free use in sensitive experiments.