Sigma-Aldrich Mutanolysin from Streptomyces globisporus ATCC 21553

Mutanolysin is a highly purified, lyophilized powder consisting of muralytic enzymes isolated from Streptomyces globisporus. With an activity level of ≥4000 units/mg protein (biuret) and chromatographic purification, it is a powerful and versatile tool for applications in microbiology, molecular biology, and enzymatic research.

General Description:

Mutanolysin consists of two primary lytic enzymes and is particularly effective against Gram-positive bacteria. It cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage in the peptidoglycan layer of bacterial cell walls. Its carboxy-terminal domains aid in the recognition and binding of specific bacterial cell wall polymers.

Key Features:

1. High Activity:
   • ≥4000 units/mg protein (biuret) ensures high efficacy for cell wall lysis.
   • Capable of lysing Listeria, Lactobacillus, Lactococcus, and other Gram-positive bacteria.
2. Purity:
   • Chromatographically purified for consistent and reliable results.
3. Gentle Lysis:
   • Provides gentle cell lysis for preserving easily degradable biomolecules like RNA and DNA.
   • Ideal for isolating intact DNA, RNA, and other biomolecules without harsh chemical treatment.
4. Lyophilized Form:
   • Contains Ficoll® and sodium succinate buffer salts for stability and easy handling.
   • Long shelf life under proper storage conditions.

Applications:

1. Microbiological Research:
   • Lysis of Gram-positive bacterial cell walls for physiological and biochemical studies.
   • Investigation of peptidoglycan structures and bacterial wall compositions.
2. Molecular Biology:
   • Formation of spheroplasts for DNA isolation.
   • RNA isolation with minimal degradation, ensuring high-quality samples for downstream applications.
3. Protoplast Formation:
   • Generation of bacterial protoplasts from dairy streptococci and Group B streptococci for genetic and proteomic research.
4. Biotechnology and Enzymatic Studies:
   • Functional studies involving enzymatic activity on bacterial substrates.
   • Evaluation of antimicrobial treatments targeting bacterial cell walls.
5. Dental Research:
   • Potential application in controlling dental caries by targeting bacterial pathogens.

Biochemical/Physiological Actions:

• Mutanolysin is an N-acetylmuramidase, similar to lysozyme.
• Cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage in bacterial peptidoglycan.
• Effective against Streptococcus, Listeria, and various Gram-positive bacteria.

Unit Definition:

One unit of mutanolysin activity produces a ΔA600 of 0.01 per minute at pH 6.0 and 37 °C in a 1 mL reaction using a suspension of Streptococcus faecalis cell wall as the substrate.

Specifications:

Available Pack Sizes:

Storage and Stability:

• Store the lyophilized powder at 2–8 °C in a dry environment.
• Reconstituted enzyme solutions should be stored at –20 °C for long-term stability.

The Sigma-Aldrich Mutanolysin from Streptomyces globisporus is a powerful and reliable enzyme for researchers working with bacterial cell walls. Its versatility and precision make it indispensable for applications in microbiology, molecular biology, and biochemical research.