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Sigma-Aldrich Immersion oil for microscopy
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viscosity: 100-120 mPa.s(20 °C)
density: 1.025 g/mL at 20 °C
Specifications:
| Application | microscopy | ||
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Laboratory grade | ||
General description
Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to approximate the refractive index of glass as closely as possible. It is used in conjunction with an objective lens to enhance the resolving power.
Application
Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, haematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.
Principle
Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.
- Pack Size: 50 mL 250 mL 1 LIter