Sigma-Aldrich Human Serum

Sigma-Aldrich™ Human Serum (from clotted whole blood, male AB donors, USA origin) is a high-quality, sterile-filtered serum optimized for advanced cell culture and regenerative medicine applications. Derived from clotted whole blood rather than plasma, this serum retains key biomolecules that support cell viability, differentiation, and expansion—particularly for stem cells and immune cells.

The use of male AB donors minimizes antibody interference, making it broadly compatible across cell types and reducing immunogenic risk. With endotoxin levels ≤10 EU/mL and hemoglobin content ≤25 mg/dL, this product is validated for use in sensitive applications such as mesenchymal stem cell (MSC) expansion, natural killer (NK) cell cultures, islet cell preparation, and limbal epithelial grafting.

Key Features

• AB Clotted Whole Blood Source: Human serum derived from clotted whole blood (not plasma), retaining clotting-related growth factors and matrix components.
• Sterile-Filtered: Triple-filtered to ensure sterility for aseptic applications.
• Endotoxin Tested: Stringent control ensures ≤10 EU/mL endotoxin for compatibility with stem and immune cells.
• Broad Application Compatibility: Suitable for both mammalian and stem cell culture systems.
• Human-Origin and Antibody-Minimized: Sourced from male AB donors, minimizing alloimmune interactions.

Applications

• Stem Cell Expansion:
  • Mesenchymal stem cells (MSCs) from adipose tissue or bone marrow.
  • Limbal epithelial stem cell graft development and transplantation.
• Immune Cell Culture:
  • Ex vivo expansion of NK cells from peripheral blood.
• Islet Cell Preparation:
  • Pre-transplant culture of human pancreatic islets.
• Tissue Engineering & Cell Therapy:
  • GMP-compatible expansion systems (research use only).
  • Clinical model development in regenerative medicine.

Specifications

Packaging and Safety Notes

• Packaging Variability: Bottle type and shrink wrap presence may vary due to supply constraints. These changes do not affect sterility or integrity.
• Visual Appearance: Cloudiness is normal due to lipid content from pooled serum; clear, lipid-free human serum is not available.
• Biosafety Notice: All donors are ELISA-tested and found non-reactive for HIV, Hepatitis B, and C. Despite this, all human-derived materials should be handled as potentially infectious in compliance with biosafety guidelines.

Regulatory Disclaimer

• For Research Use Only. Not for diagnostic, therapeutic, or clinical use in humans or animals.

Sigma-Aldrich™ Human Serum (from male AB clotted whole blood) is an advanced, high-quality reagent suitable for research requiring human-specific conditions, especially in stem cell biology, immunotherapy, and cell-based regenerative medicine. With superior lot consistency and verified sterility, it is a trusted component in protocols where animal serum alternatives are unsuitable or undesired.