Sigma-Aldrich Chloroform : Isoamyl alcohol 24:1, suitable for nucleic acid purification

Sigma-Aldrich Chloroform:Isoamyl Alcohol (24:1)

Sigma-Aldrich Chloroform:Isoamyl Alcohol (24:1) is a high-purity organic reagent widely used in molecular biology for nucleic acid and protein extraction. Its optimized composition ensures effective phase separation during DNA, RNA, and protein isolation processes, making it a reliable choice for research applications.

Key Features

1. High-Purity Reagent:
   • Optimized 24:1 ratio of chloroform to isoamyl alcohol.
   • Stabilized with 0.6–1.0% ethanol for enhanced stability and usability.
2. Versatile Applications:
   • Suitable for the extraction and precipitation of DNA, RNA, and proteins.
   • Effective in phenolic oxidation and separating phenolics from aqueous phases.
3. Molecular Biology Grade:
   • Tested for suitability in nucleic acid purification protocols.
   • Ensures high-quality results for sensitive research workflows.

Applications

1. Nucleic Acid Extraction:
   • Used to extract genomic DNA from bacteria, Daphnia spp, and salmon sperm.
   • Facilitates a single DNA precipitation step for high-quality DNA extraction.
2. RNA Isolation:
   • Applied in RNA extraction from various tissues, including brown and white adipose tissues.
3. Protein Extraction:
   • Used for protein separation and RNAse treatment in molecular workflows.
4. Phenolic Oxidation:
   • Efficiently separates phenolics from aqueous solutions during phenol-based extractions.
5. Modified CTAB Method:
   • Plays a crucial role in extracting total RNA from plant tissues, such as Impatiens balsamina.

Specifications

Advantages

• Phase Separation Efficiency:
  • Optimized ratio ensures clean phase separation for efficient nucleic acid recovery.
• Stabilized Composition:
  • Ethanol stabilizer maintains reagent integrity and usability over time.
• Molecular Biology Grade:
  • Ensures compatibility with sensitive DNA, RNA, and protein workflows.

Safety Information

• Hazards: Classified as toxic and flammable; handle with caution.
• Precautions:
  • Use in a well-ventilated area or fume hood.
  • Wear appropriate personal protective equipment (gloves, goggles, lab coat).
• Storage: Store in a cool, dry place, away from heat and ignition sources.

Sigma-Aldrich Chloroform:Isoamyl Alcohol (24:1) is a reliable, high-purity reagent designed for DNA, RNA, and protein extraction. Its stabilized formulation and optimized composition make it an essential tool for molecular biology applications requiring clean and efficient phase separation.