Sigma-Aldrich 2,4-Dinitrophenylhydrazine reagent grade, 97%

​Sigma-Aldrich™ 2,4-Dinitrophenylhydrazine (DNPH) is a high-quality analytical reagent widely used for the derivatization and detection of aldehydes and ketones, particularly in environmental monitoring, carbonyl analysis, and organic chemistry. With a minimum assay of 97% purity and verified suitability for HPLC analytical testing, DNPH is an essential reagent in laboratories working with carbonyl compounds, oxidative stress biomarkers, and atmospheric pollutant analyses.

DNPH reacts with aldehydes and ketones to form stable 2,4-dinitrophenylhydrazones, allowing sensitive detection using chromatographic or spectrophotometric techniques. This reagent is also commonly used for carbonylation assays in protein research and pharmaceutical analysis.

Key Features

• Reagent grade, 97% purity suitable for analytical and spectroscopic applications.
• Highly reactive hydrazine derivative for aldehyde and ketone derivatization.
• Suitable for HPLC-based analytical testing.
• Contains ≥33% water to stabilize the dry reagent.
• Reliable reagent for carbonyl detection, atmospheric sampling, and pharmaceutical quantification.
• Backed by Sigma-Aldrich’s high purity and QC standards.

Applications

1. Detection of Aldehydes & Ketones

• Forms hydrazone derivatives used for identification and quantification.
• Widely applied in environmental chemistry and atmospheric pollution analysis (e.g., carbonyl monitoring in air).

2. Protein Carbonylation Studies

• DNPH is the standard reagent for detecting protein carbonyl groups, which serve as biomarkers of oxidative stress.
• Used in biochemical research exploring protein oxidation, aging, inflammation, and disease pathways.

3. Pharmaceutical & Chemical Analysis

• Acts as a colorimetric reagent for prednisone and related corticosteroids.
• Used in quality control assays and stability testing.

4. Structural & Spectroscopic Research

• Molecular structure, hydrogen bonding, and thermal degradation properties make DNPH a reference material in chemistry research.

Technical Specifications

Safety & Handling Notes

• DNPH is a hydrazine derivative and is potentially mutagenic; handle with appropriate PPE.
• Avoid heating the dry solid; DNPH can be thermally unstable.
• Use in well-ventilated areas and follow all SDS guidelines.

Ordering Information

Sigma-Aldrich™ 2,4-Dinitrophenylhydrazine (DNPH), 97% reagent grade, is a critical analytical reagent for laboratories conducting aldehyde/ketone derivatization, carbonyl stress biomarker analysis, pharmaceutical testing, and environmental monitoring. Its high purity, compatibility with HPLC methods, and reliable performance make it an indispensable component of chemical, biochemical, and environmental workflows.