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Specifications:
| Application | Protein Biology, Size Exclusion Chromatography | ||
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Chemical | Forms | Solid |
| Product Brand | Cytiva | ||
| Product Grade | Molecular Biology | ||
Sephadex™ G-25 is a widely used gel filtration chromatography resin designed for rapid desalting, buffer exchange, and contaminant removal in industrial and laboratory applications. This cross-linked dextran-based matrix efficiently separates small molecules from larger biomolecules, such as proteins, peptides, and nucleic acids, through size exclusion chromatography (SEC).
Sephadex™ G-25 provides excellent recovery with minimal dilution, making it ideal for sample preparation before high-resolution chromatography or other downstream processes.
Key Features and Benefits
- Desalting and Buffer Exchange in One Step
- Removes salts and small contaminants while transferring biomolecules into a new buffer.
- No additional purification steps required.
- High Recovery with Minimal Dilution
- Ensures excellent sample yield while maintaining concentration.
- Ideal for preparing samples before ion exchange or affinity chromatography.
- BioProcess Resin for Industrial Applications
- Scalable for biopharmaceutical and large-scale production.
- Compatible with industrial chromatography systems.
- Versatile Particle Size Options
- Available in different bead sizes to accommodate various flow rates and separation needs:
- Superfine (20-50 µm): High-resolution separations with shorter diffusion distances.
- Fine (20-80 µm): Suitable for general lab-scale applications.
- Medium (50-150 µm): Optimized for large-scale separation with high flow rates.
- Coarse (>100 µm): Designed for bulk separations at high flow rates with low operating pressures.
- Available in different bead sizes to accommodate various flow rates and separation needs:
- Wide pH Stability (2-13)
- Compatible with a broad range of buffers and conditions.
- Suitable for both acidic and basic environments.
- Prepacked Column Options
- Available in HiPrep™ desalting columns for fast and convenient use.
Applications
- Desalting of Proteins and Peptides
- Removal of excess salts after protein purification.
- Buffer Exchange
- Transfer biomolecules into a different buffer system for further experiments.
- Removal of Small Contaminants
- Efficiently eliminates unwanted small molecules such as nucleotides, salts, and detergents.
- Sample Preparation for High-Resolution Chromatography
- Prepares samples before ion exchange, affinity chromatography, or hydrophobic interaction chromatography.
- Biopharmaceutical Processing
- Used in large-scale protein purification and drug formulation.
- DNA & RNA Purification
- Removal of unincorporated nucleotides from nucleic acid samples.
Technical Specifications
| Matrix | Cross-linked dextran |
|---|---|
| Fractionation Range | 1×10² – 5×10³ dextrans, 1×10³ – 5×10³ globular proteins |
| pH Range | 2 - 13 |
| Storage Temperature | 4 - 30 °C |
| Chemical Stability | Stable in most aqueous buffers and common laboratory solvents |
Available Variants
| Description | Type | Particle Size | Exclusion Limit | Pack Size | Catalog Number |
|---|---|---|---|---|---|
| Sephadex™ G-25 Superfine | Solid | 20 - 50 µm | >5 × 10³ | 100 g | 17-0031-01 |
| Sephadex™ G-25 Fine | Solid | 20 - 80 µm | >5 × 10³ | 100 g | 17-0032-01 |
| Sephadex™ G-25 Fine | Solid | 20 - 80 µm | >5 × 10³ | 500 g | 17-0032-02 |
| Sephadex™ G-25 Medium | Solid | 50 - 150 µm | >5 × 10³ | 100 g | 17-0033-01 |
| Sephadex™ G-25 Medium | Solid | 50 - 150 µm | >5 × 10³ | 500 g | 17-0033-02 |
| Sephadex™ G-25 Coarse | Solid | >100 µm | >5 × 10³ | 100 g | 17-0034-01 |
| Sephadex™ G-25 Coarse | Solid | >100 µm | >5 × 10³ | 500 g | 17-0034-02 |
Usage Guidelines
- Equilibration:
- Equilibrate the Sephadex™ G-25 resin with the desired buffer before use.
- Sample Application:
- Apply the sample onto the column or packed resin bed.
- Separation Process:
- Allow larger biomolecules to pass through while retaining smaller contaminants.
- Collection:
- Collect the purified fractions, ensuring high recovery of the desired biomolecules.
Storage and Handling
- Store between 4-30°C in a dry environment.
- Avoid excessive compression or drying of the resin to maintain integrity.
- For long-term storage, keep in an appropriate buffer with antimicrobial agents if necessary.
Sephadex™ G-25 is an industry-leading gel filtration medium designed for efficient desalting, buffer exchange, and contaminant removal. With multiple particle size options, it supports a wide range of laboratory and industrial-scale applications. Its high recovery, minimal dilution, and excellent reproducibility make it an essential tool in protein purification, biopharmaceutical processing, and molecular biology research.
- Pack Size: superfine, 20 - 50 µm, 100g Fine, 20 - 80 µm, 100g fFine, 20 - 80 µm, 500g medium, 50 - 150 µm, 100g medium. 50 - 150 µm, 500g Coarse Solid, >100 µm, 100g Coarse Solid, >100 µm, 500g