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Virplex® Monkeypox RealTime PCR Kit, 96 Rxn
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Targets: F3L gene (MPXV-specific), E9L gene (Orthopoxvirus genus), Human RNAse P (internal control)
Specifications:
| Application | qPCR, Molecular Diagnostics | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® Monkeypox RealTime PCR Kit is a multiplex real-time PCR assay designed for the one-step detection of monkeypox virus (MPXV) nucleic acids from human skin lesion samples. Featuring a double-target design, the kit provides both specific detection of MPXV (F3L gene) and generic detection of Orthopoxvirus genus (E9L gene), ensuring sensitivity and reliability in suspected cases.
Key Features
- Double target assay – MPXV-specific detection (F3L gene) and Orthopoxvirus genus detection (E9L gene).
- Multiplex PCR format – one single reaction tube per sample.
- Internal human RNAse P control – monitors sample quality, nucleic acid integrity, and amplification set-up.
- Compatible with multiple qPCR channels – FAM, Cy5, HEX (VIC).
- Fast results – turnaround time of ~1 h 30 min.
- Lyophilized reagents – master mix and positive control for long-term stability and reduced cold chain logistics.
- New pre-dispensed format – 96 × 0.1 mL tear-off plates with 12 break-apart 8-tube strips for ease of use.
- Research Use Only (RUO) – optimized for laboratory research and outbreak monitoring.
Applications
- Detection of Monkeypox virus (MPXV) in suspected cases.
- Differentiation of Orthopoxvirus vs. specific MPXV infections.
- Outbreak investigation, epidemiological tracking, and surveillance.
- Research use in molecular virology laboratories.
- Training and validation of monkeypox molecular diagnostic methods.
Product Details
| Property | Value |
|---|---|
| Product Name | Virplex® Monkeypox RealTime PCR Kit |
| Reference | RTPCR018-LPD-R |
| Nº Tests | 96 |
| Class | RUO – Research Use Only |
| Format | Multiplex real-time PCR kit |
| Sample Type | Human skin lesion samples (vesicular smear on transport medium or dry swab) |
| Targets | F3L gene (MPXV-specific), E9L gene (Orthopoxvirus genus), Human RNAse P (internal control) |
| Interpretation | FAM (Monkeypox virus – specific), HEX/VIC (Orthopoxvirus), Cy5 (Internal control) |
| Time to Results | ~1 h 30 min |
| Kit Components | Single mix, reconstitution solution, positive control, negative control |
| Format | Pre-dispensed lyophilized master mix in 96 × 0.1 mL 8-tube strip plates |
| Control Concentration | 10,000–20,000 copies/µL |
| Storage | Lyophilized, stable at ambient conditions |
Compatibility
Extraction Systems:
- BioMérieux NucliSENS® easyMag®
- Roche MagNA Pure System
- TANBead Maelstrom 4800 and 9600
- Thermo Fisher KingFisher Flex
- Bruker GenoXtract 12
- (and other compatible systems)
Real-Time PCR Thermocyclers:
- Bio-Rad CFX96 Touch™
- Roche LightCycler® 96, LightCycler® 480, COBAS® z480
- Agilent Aria Mx
- Thermo Fisher ABI 7500 / 7500 Fast
- Azure Cielo 6
- (and other FAM/HEX/Cy5-compatible cyclers)
Related Products
- Virplex® MPOX Clades RealTime PCR Kit – RTPCR034-R (Clade I/II differentiation)
- AMPLIRUN® Monkeypox Virus DNA Control – MBC146-R
- AMPLIRUN® Total Monkeypox Virus Control (Swab) – MBTC032-R
The Virplex® Monkeypox RealTime PCR Kit provides a robust, multiplex molecular assay for the detection of monkeypox virus with internal controls for sample integrity and inhibition monitoring. With its double-target design, pre-dispensed lyophilized format, and wide system compatibility, it is a reliable tool for outbreak response, epidemiological surveillance, and molecular virology research.