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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Enzymes and Substrates | Forms | Lyophilized |
| Product Brand | ROCHE | ||
| Product Grade | Molecular Biology | ||
Roche™ Pronase is a high-quality non-specific protease mixture derived from Streptomyces griseus, designed for comprehensive protein degradation in molecular biology and biochemical applications.
With its broad substrate specificity, Pronase efficiently hydrolyzes proteins into amino acids and small peptides, making it ideal for DNA/RNA extraction, tissue dissociation, and protein analysis workflows.
Supplied as a lyophilized powder, this enzyme provides excellent stability, high activity, and reproducible performance for demanding laboratory applications.
Key Features and Benefits
Broad Proteolytic Activity
Pronase exhibits wide substrate specificity, enabling the breakdown of virtually all proteins into their constituent amino acids.
Optimized for Molecular Biology Applications
Highly suitable for DNA and RNA isolation workflows, including plasmid and phage DNA extraction.
High Enzymatic Activity
Approximately 1270 PU/mg (casein substrate at 40°C, pH 7.5), ensuring efficient and rapid protein digestion.
Minimal Nuclease Contamination
Very low DNase and RNase activity (<0.002 U/mg), preserving nucleic acid integrity during extraction.
Enhanced Stability with Calcium Ions
Formulated with calcium acetate to improve enzyme stability and resistance to heat inactivation.
Flexible Usage Across Applications
Effective in cell culture, histochemistry, glycoprotein processing, and tissue dissociation workflows.
Applications
- DNA and RNA extraction (plasmid, genomic, and phage DNA)
- Protein digestion and hydrolysis
- Tissue dissociation in cell culture (with collagenase/trypsin)
- Histochemistry and cellular analysis
- Glycopeptide production from glycoproteins
- Zebrafish embryo dechorionation
- Protein removal in nucleic acid purification workflows
Technical Specifications
| Parameter | Specification |
|---|---|
| Biological Source | Streptomyces griseus |
| Enzyme Type | Non-specific protease |
| Form | Lyophilized powder |
| Color | White |
| Activity | ~1270 PU/mg |
| Optimum Temperature | 35–40 °C |
| Optimum pH | 7.0–8.0 |
| pH Range | 6.0–9.0 |
| Solubility | 10–20 g/L (water) |
| Working Concentration | 0.5–2 mg/mL |
| DNase Activity | <0.002 U/mg |
| RNase Activity | <0.002 U/mg |
| Storage (Dry) | 2–8 °C |
| Storage (Solution) | -15 to -25 °C |
Ordering Information
| Pack Size | Product Code |
|---|---|
| 1 g | 10165921001 |
| 5 g | 11459643001 |
Preparation and Handling
- Reconstitute in distilled water to prepare stock solutions (10–20 mg/mL)
- Recommended working concentration: 0.5–2 mg/mL
- Calcium ions (0.01–0.1 M) enhance stability and protect against heat inactivation
- Store working solutions at -15 to -25 °C for optimal stability
Performance Characteristics
Pronase demonstrates exceptional efficiency in protein degradation due to its broad specificity. Compared to enzymes such as trypsin, it provides superior performance in:
- Complete protein digestion
- Fibroblast cell detachment and dispersion
- Generation of single-cell suspensions
Its ability to function across a wide pH range and temperature window makes it a versatile enzyme for both routine and advanced laboratory workflows.
Unique Advantages
- Broad-spectrum protease activity for complete protein hydrolysis
- Minimal nuclease contamination for nucleic acid workflows
- Stabilized formulation with calcium acetate
- Highly versatile across molecular biology and cell culture applications
- Reliable performance from a globally recognized manufacturer
Roche™ Pronase from Streptomyces griseus is a powerful and versatile proteolytic enzyme designed for laboratories requiring efficient and reliable protein degradation. Its broad specificity, high activity, and compatibility with nucleic acid workflows make it an essential reagent for molecular biology, biochemistry, and cell culture applications.
- Pack Size: 1 g 5 g