Sigma-Aldrich Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit

The Sigma-Aldrich Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit is a high-performance assay designed for the quantitative detection of FABP2 (Fatty Acid-Binding Protein 2) in various biological samples, including serum, plasma, urine, and cell culture supernatants. This kit is a robust tool for studying lipid metabolism, adipogenesis, and diseases such as diabetes and insulin resistance.

Key Features

1. High Sensitivity and Precision:
   • Sensitivity: Detects as low as 25 pg/mL.
   • Inter-Assay CV: <10%.
   • Intra-Assay CV: <12%.
2. Wide Sample Compatibility:
   • Suitable for human serum, plasma, urine, and cell culture supernatants.
3. Comprehensive Kit Components:
   • Packaged in a 96-well format (12 strips x 8 wells) for flexibility in sample testing.
4. Reliable Detection Method:
   • Uses a colorimetric detection system for precise quantification.
5. Robust Antibody Pair:
   • The kit includes an antibody pair specific to human intestinal FABP2 (I-FABP), ensuring specificity and sensitivity.
6. Convenient Handling:
   • Shipped on wet ice to maintain reagent stability.
   • Stored at −20°C for extended usability.

Applications and Use Cases

1. Clinical and Disease Research:

• Diabetes and Insulin Resistance:
  • Quantify FABP2 levels to study its role as a candidate gene for diabetes.
• Lipid Metabolism Disorders:
  • Investigate the function of FABP2 in lipid absorption and metabolism.
• Obesity and Adipogenesis:
  • Explore the involvement of FABP2 in fat storage and energy homeostasis.

2. Nutritional and Gastrointestinal Studies:

• Dietary Fat Absorption:
  • Evaluate FABP2 activity in response to different dietary interventions.
• Enterocyte Function Research:
  • Study the role of FABP2 in the intestinal absorption of long-chain fatty acids.

3. Pharmaceutical and Drug Development:

• Therapeutic Targets:
  • Assess the effect of drugs targeting FABP2 in metabolic diseases.
• Biomarker Discovery:
  • Use FABP2 as a biomarker for gastrointestinal health and lipid metabolism.

4. Cell Biology and Molecular Studies:

• Cell Culture Experiments:
  • Analyze FABP2 expression in cultured cells to study intracellular fatty acid transport.

Kit Components

• Pre-Coated 96-Well Plate: Pre-coated with an antibody specific to FABP2.
• FABP2 Standards: For generating the standard curve for sample quantification.
• HRP-Conjugated Detection Antibody: Ensures specific and sensitive detection.
• Sample and Standard Diluent: Optimizes sample preparation for accurate results.
• Wash Buffer: Removes unbound components during washing steps.
• TMB Substrate Solution: Produces a colorimetric signal proportional to FABP2 concentration.
• Stop Solution: Stabilizes the reaction for consistent optical density (OD) readings.
• Instruction Manual: Step-by-step protocol for assay execution.

Performance Specifications

Storage and Stability

• Unopened Product: Store at −20°C.
• Opened Product: Follow manufacturer instructions for reagent stability.
• Shipped on wet ice to preserve reagent integrity.

Biological Significance of FABP2

Fatty Acid-Binding Protein 2 (FABP2):

• Function:
  • Intracellular protein responsible for absorbing and transporting long-chain fatty acids in intestinal enterocytes.
  • Plays a critical role in lipid metabolism and energy regulation.
• Clinical Relevance:
  • A candidate gene for diabetes and insulin resistance.
  • Implicated in obesity and related metabolic syndromes.
• Gene Information:
  • Human FABP2 gene is located at chromosome 4q28–q31.

Why Choose This Kit?

1. Comprehensive Research Tool: Suitable for multiple biological sample types and applications.
2. Validated Performance: High sensitivity and reproducibility for accurate results.
3. User-Friendly Design: Pre-coated plates and ready-to-use reagents streamline the workflow.
4. Reliable Results: Backed by Sigma-Aldrich’s quality assurance and validated protocols.

The Sigma-Aldrich Human FABP2 ELISA Kit is a powerful tool for researchers studying lipid metabolism, diabetes, and obesity. With its high sensitivity, robust antibody design, and versatile sample compatibility, this kit ensures reliable and reproducible results in both basic and applied research settings.