Thermo Scientific™ dNTP/dUTP Mix, 2 mM, 1mL

High-Purity dNTP/dUTP Mix for Uracil-Incorporating PCR and Reverse Transcription

The Thermo Scientific™ dNTP/dUTP Mix is a high-quality, ready-to-use solution containing dATP, dCTP, and dGTP (2 mM each) and dUTP (4 mM). This optimized mix is ideal for molecular biology workflows requiring uracil substitution for dTTP, such as uracil-DNA glycosylase (UDG)-based carryover prevention in PCR. With >99% purity, the dNTP/dUTP Mix supports consistent and contamination-free amplification.

Key Features

• Substitution-Ready Mix: Use in place of standard dNTPs to incorporate dUTP in PCR
• High Purity: Each nucleotide is HPLC-verified to be >99% pure
• Contamination-Free: No detectable nuclease activity or human/E. coli DNA
• Exceptional Stability:
  • Stable for up to 100 freeze-thaw cycles
  • Maintains ≥95% triphosphate form after 7 weeks at room temperature
  • Maintains ≥90% triphosphate form after 30 PCR cycles

Applications

• PCR and qPCR (with Taq DNA Polymerase only)
• cDNA Synthesis and RT-PCR
• DNA Sequencing
• UDG-based carryover contamination prevention protocols

Note: Not compatible with proofreading enzymes or any enzyme mixes containing proofreading activity.

Specifications

Ordering Information

The Thermo Scientific™ dNTP/dUTP Mix is a reliable, ultra-pure nucleotide solution optimized for PCR protocols that require dUTP substitution. Ideal for carryover prevention and sensitive applications in molecular biology, this mix improves workflow accuracy and reproducibility in a convenient, ready-to-use format.