Pierce™ Premium Grade TCEP-HCl

Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl is a high-purity, thiol-free disulfide reducing agent. It is specially formulated for applications where product integrity, risk minimization, and reliability are critical. This premium grade product undergoes enhanced quality testing, batch-specific characterization, and lot retention to ensure superior performance and consistency.

Key Features:

1. High-Quality Disulfide Reducing Agent:
   • TCEP (Tris(2-carboxyethyl)phosphine hydrochloride) effectively reduces protein and peptide disulfide bonds over a wide pH range.
   • Thiol-free and odorless, unlike DTT or BME, making it convenient for bench-top use.
2. Premium Grade Characteristics:
   • Increased analytical testing and characterization for quality assurance.
   • Enhanced batch-specific manufacturing documentation.
   • Extensive lot retention to ensure reproducibility in future testing.
   • Change Control Notification (CCN) service for process consistency.
3. Performance Advantages:
   • Odorless: Unlike BME or DTT, TCEP is free from unpleasant odors.
   • Air-Stable: No special precautions required for handling, storage, or use.
   • Efficient and Compatible: Effective at low concentrations (5–50 mM) and does not require removal before most downstream applications (e.g., His-tagged protein purification, maleimide conjugation).

Applications:

1. Protein and Peptide Reduction:
   • Selectively and completely reduces water-soluble disulfides.
2. Sample Preparation:
   • Ideal for reducing agents in mass spectrometry, protein assays, or purification protocols.
3. Conjugation Workflows:
   • Suitable for maleimide conjugation and other chemical modification processes.
4. General Laboratory Use:
   • Efficient and odor-free reducing agent for various research needs.

Advantages of TCEP-HCl Over DTT or BME:

Specifications:

Catalog Numbers and Quantities:

Handling and Storage:

• Storage Conditions: Store at room temperature in a sealed container to prevent oxidation.
• Shipping: Shipped at ambient temperature.

For research applications requiring a reliable and high-quality reducing agent, Pierce™ Premium Grade TCEP-HCl is an excellent choice, offering superior stability and compatibility.