PEIpro® DNA transfection reagent for virus production

PEIpro® is a next-generation linear polyethylenimine (PEI)-based transfection reagent developed specifically for high-efficiency DNA delivery into suspension-adapted mammalian cells. It is ideal for transient gene expression (TGE) applications in research and commercial-scale viral vector production, such as lentivirus and AAV, as well as recombinant protein manufacturing.

Designed to meet the demanding requirements of bioprocess development and GMP-compatible production workflows, PEIpro® supports high reproducibility, safety, and scalability from milliliter-scale assays to bioreactor-based production runs.

Key Features

• Optimized for Virus Production
  PEIpro® offers superior performance for the production of lentiviral and adeno-associated viral (AAV) vectors, achieving high transfection efficiency and viral titers in mammalian cell cultures such as HEK293, HEK293T, and derivative lines.
• Scalable from R&D to Production
  Its linear, fully synthetic formulation allows seamless scalability across various volumes and platforms—from small shake flasks to large stirred-tank bioreactors—without protocol modification.
• High Quality and Lot-to-Lot Consistency
  Each batch of PEIpro® is rigorously tested and certified for:
  • Transfection efficiency in HEK293 cells
  • Sterility
  • Endotoxin levels
  • Absence of animal-derived materials
• Animal-Free, GMP-Compatible Formulation
  PEIpro® is produced under an ISO 9001-certified quality system, supporting regulatory compliance and minimizing risks associated with biologics manufacturing.
• Ready-to-Use Format
  Pre-sterilized and conveniently packaged, PEIpro® simplifies workflow and saves preparation time in high-throughput or critical production settings.

Typical Applications

• Lentivirus and AAV production for gene therapy research
• Recombinant protein expression in suspension cells
• Transient transfection in HEK293 and CHO cells
• Genome engineering using CRISPR-Cas9 systems
• DNA and siRNA delivery

Ordering Information

Larger volumes and bulk packaging options are available upon request.

Storage and Stability

• Store at 2–8°C
• Do not freeze
• Shelf life: 12 months from the date of manufacture
• For research or further manufacturing use only. Not intended for diagnostic or therapeutic applications in humans or animals.

Sartorius PEIpro® represents a powerful, versatile, and scalable solution for researchers and manufacturers seeking reliable transient transfection of mammalian cells. Whether used in R&D labs or in upstream bioprocess development for gene therapy products, PEIpro® consistently delivers high transfection efficiency, superior viral vector yields, and process robustness. Its animal-origin free composition and validated quality make it the reagent of choice for both high-throughput research and clinical-grade vector production.