Isolation of total DNA from Gram-positive and Gram-negative bacteria, yeast, and fungi – MN Bead Tubes Type B included

NucleoSpin Microbial DNA

• Suitable for a large variety of starting materials
• MN Bead Tubes Type B for efficient lysis included – compatible with many disruption devices
• Fast and easy procedure – Liquid Proteinase K included, no additional enzyme required

NucleoSpin Microbial DNA procedure

NucleoSpin Microbial DNA is designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi).

The NucleoSpin Microbial DNA kit utilizes MN Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K, and a common disruption device e.g., swing mill, in order to lyse different sample materials with one procedure. The procedure is fast, simple and reliably high DNA yields from a wide range of microbial samples can be obtained. Alternatively MN Bead Tubes can be ordered separately.

Appropriate DNA binding conditions to the NucleoSpin Microbial DNA Columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer MG) to the lysate. Contaminants are removed by two efficient washing steps. Afterwards, DNA is eluted with a slight alkaline buffer and is ready-to-use for subsequent reactions.

Comparison of preparation times

Efficient DNA recovery from different microorganisms

DNA was isolated with the NucleoSpin Microbial DNA kit and MN Bead Tube Type B (included in the kit) or MN Bead Tube Type C (see ordering information). 100 ng DNA per prep were analyzed by agarose gel electrophoresis, showing high molecular DNA, without RNA contamination or DNA degradation.

The prep time of NucleoSpin Microbial DNA kit was compared with competitor products by processing Bacillus subtilis, Escherichia coli, and Saccharomyces cerevisiae. The NucleoSpin Microbial DNA kit shows shorter prep times in all three cases. 

Various applications are possible in combination with the MN Bead Tubes

DNA was successfully isolated from the above mentioned microorganisms by the NucleoSpin Microbial DNA kit.

The MN Bead Tubes were tested for the mentioned sample materials on a Retsch Swingmill MM300, operating at highest frequency (30 Hertz). The time and frequency might have to be optimized for other disruption devices, as well as other sample materials.