MyBioSource Human Neurofilament, Light Polypeptide (NEFL) ELISA Kit

The MyBioSource NEFL ELISA Kit is a highly sensitive sandwich ELISA designed for the detection and quantification of Neurofilament, Light Polypeptide (NEFL) in human biological samples. This kit is suitable for neuroscience research and is optimized for serum, plasma, tissue homogenates, and other biological fluids. With a wide detection range and excellent specificity, it enables researchers to study neuronal health and neurodegenerative conditions with confidence.

Key Features:

1. High Sensitivity and Wide Detection Range:
   • Sensitivity: 6.2 pg/mL.
   • Detection range: 15.6–1,000 pg/mL.
2. Micro-Volume Sample Efficiency:
   • Requires only 15–25 µL of sample per well.
3. Reproducibility:
   • Intra-Assay CV: <10%.
   • Inter-Assay CV: <12%.
4. Double-Antibody Sandwich Format:
   • Ensures high specificity by using a pre-coated monoclonal antibody and biotinylated detection antibody.
5. Colorimetric Detection:
   • Utilizes an HRP-TMB enzyme-substrate system, allowing easy quantification at 450 nm.
6. Comprehensive Kit Components:
   • Includes pre-coated 96-well plate, detection reagents, buffers, standards, and adhesive plate covers.
7. ISO-Certified Manufacturing:
   • Produced in laboratories certified under ISO 9001:2008 and ISO 13485:2003.

Specifications:

Applications:

1. Neurodegenerative Disease Research:
   • Quantifies NEFL levels to study conditions like Alzheimer's, Parkinson's, ALS, and multiple sclerosis.
2. Biomarker Discovery:
   • Measures NEFL as a biomarker for axonal damage and neuronal health.
3. Basic Neuroscience Research:
   • Investigates the role of neurofilament proteins in neuronal structure and function.
4. Drug Development:
   • Evaluates the efficacy of neuroprotective therapies in preclinical studies.
5. Diagnostics Development:
   • Supports research on potential diagnostic applications of NEFL for neurological disorders.

Assay Principle:

1. Double-Antibody Sandwich Format:
   • A pre-coated antibody binds NEFL in the sample.
   • A biotin-conjugated detection antibody binds to the captured NEFL antigen.
   • Streptavidin-HRP binds to the biotin-conjugated detection antibody, completing the complex.
2. Enzyme-Substrate Reaction:
   • TMB substrate reacts with HRP to produce a color change proportional to the NEFL concentration.
3. Quantification:
   • Absorbance is measured at 450 nm, and NEFL levels are determined using a standard curve.

Kit Components:

• Pre-coated 96-well plate.
• Standards for generating a calibration curve.
• Biotinylated detection antibody.
• Streptavidin-HRP conjugate.
• Wash buffer.
• TMB substrate.
• Stop solution.
• Adhesive plate covers.

Advantages:

1. High Sensitivity:
   • Detects low levels of NEFL, making it suitable for early-stage biomarker studies.
2. Minimal Sample Volume:
   • Requires only a small amount of sample, preserving precious specimens.
3. Comprehensive Kit:
   • Provides all necessary reagents for easy setup and execution.
4. Reliable and Reproducible:
   • Certified precision ensures consistent results across replicates and experiments.
5. Versatile Sample Compatibility:
   • Works with a wide range of biological fluids and tissue homogenates.

Storage and Handling:

• Short-Term Storage: Store reagents at 4°C for up to 1 month.
• Long-Term Storage: Store at -20°C for up to 1 year.
• Reagent Handling: Avoid repeated freeze-thaw cycles. Ensure reagents are equilibrated to room temperature before use.

Ordering Information:

Research Area:

• Neuroscience: Focused on axonal health, neuronal damage, and neurodegenerative conditions.

The MyBioSource NEFL ELISA Kit provides a powerful tool for detecting and quantifying Neurofilament, Light Polypeptide (NEFL) in human biological samples. Its high sensitivity, low sample requirement, and reproducibility make it an essential resource for researchers studying neurodegeneration and neuronal health.