Thermo Scientific™ Oxoid™ Bile Salts

The Thermo Scientific™ Oxoid™ Bile Salts is a powdered ox-bile extract that serves as a selective inhibitory agent in culture media formulations. It is commonly used to suppress the growth of non-target Gram-positive organisms, enhancing the selective isolation of Gram-negative bacteria. With a standardized formulation, this product is suitable for use in various microbiological applications, including food safety, clinical diagnostics, and environmental testing.

Key Features

1. Selective Inhibition:
   • Suppresses Gram-positive bacteria, promoting the selective growth of Gram-negative microorganisms.
2. Standardized Formulation:
   • Ensures consistent performance across multiple applications.
3. Flexible Use:
   • Normal working concentration of 0.5% w/v allows for customization in various media formulations.
4. High Yield:
   • Each vial is sufficient to supplement 500mL of medium, making it cost-effective for routine use.
5. Powder Form:
   • Supplied in an easy-to-handle powder format for precise preparation and long shelf life.

Applications

1. Food Microbiology

• Used in media formulations to detect and isolate foodborne Gram-negative pathogens, including coliforms and Salmonella species.

2. Clinical Microbiology

• Enhances the isolation of enteric pathogens from clinical samples, such as stool cultures.

3. Environmental Microbiology

• Facilitates the detection of Gram-negative bacteria in water, soil, and other environmental samples.

Specifications

Preparation Instructions

1. Determine Concentration:
   • Use at the recommended working concentration of 0.5% w/v (e.g., 2.5g per 500mL of medium).
2. Mix with Medium:
   • Add the required amount of bile salts powder to the medium during preparation.
3. Sterilization:
   • Sterilize the medium as per protocol to ensure optimal performance.

Advantages

1. Enhanced Selectivity: Promotes targeted growth by inhibiting non-target Gram-positive bacteria.
2. Reliable Performance: Standardized formulation ensures consistent results in culture media.
3. Versatile Applications: Suitable for a wide range of microbiological testing in food, clinical, and environmental settings.
4. Ease of Use: Powder form allows for precise and convenient preparation.

Storage and Stability

• Storage: Keep in a cool, dry environment away from moisture and direct sunlight.
• Shelf Life: Refer to the product label for the expiration date.

Packaging

• Product: Bile Salts
• Quantity: 250g
• Unit Size: Each

The Thermo Scientific™ Oxoid™ Bile Salts is a reliable and standardized selective inhibitory agent essential for microbiological media formulations. Its ability to suppress Gram-positive bacteria while supporting the growth of Gram-negative organisms makes it a critical component in food safety testing, clinical diagnostics, and environmental monitoring. The powdered form ensures ease of use and consistent performance, making it an indispensable tool for microbiology laboratories.