Liofilchem™ MTS™ Imipenem IMI 0.002-32, MIC Test strips

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Termini e condizioni
Garanzia di rimborso di 30 giorni
Spedizione: 2-3 giorni lavorativi

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Specifications:
Application Clinical microbiology
Storage Temperature -20°C
Product Type MIC Strips
Product Brand Liofilchem
Product Grade Microbiology grade

The Liofilchem™ MTS™ Imipenem IMI 0.002-32 MIC Test Strips are designed for precise Minimum Inhibitory Concentration (MIC) determination of imipenem against a broad range of microorganisms. These strips are ideal for detecting carbapenem resistance mechanisms in clinical and research laboratories, especially in Gram-negative bacteria.

Key Features

  1. Broad MIC Range:
    • Covers concentrations from 0.002 μg/mL to 32 μg/mL, suitable for highly resistant strains.
  2. Ease of Use:
    • Simple application on Mueller-Hinton Agar.
  3. Accurate Results:
    • Provides clear MIC endpoints within 16–20 hours of incubation.
  4. Global Standards:
    • Compliant with CLSI, EUCAST, and other international guidelines.
  5. Resistance Detection:
    • Effectively identifies carbapenemase-producing Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter spp.

Applications

ApplicationDetails
Clinical DiagnosticsIdentification of imipenem-resistant pathogens.
Resistance Mechanism DetectionScreening for carbapenemase production in Gram-negative bacteria.
Epidemiological StudiesSurveillance of antimicrobial resistance trends.
Pharmaceutical ResearchAssessment of new carbapenem antibiotics.

Testing Procedure

  1. Preparation:
    • Prepare a 0.5 McFarland standard suspension of the test organism.
    • Inoculate the suspension evenly on a Mueller-Hinton Agar plate.
  2. Application:
    • Place the Liofilchem Imipenem MIC Test Strip on the agar surface using sterile forceps.
  3. Incubation:
    • Incubate the plate aerobically at 35–37°C for 16–20 hours.
  4. Interpretation:
    • Determine the MIC by observing the point where the elliptical zone of inhibition intersects the strip.

Technical Specifications

FeatureDetails
Active CompoundImipenem
Concentration Range0.002–32 μg/mL
Compatible MediaMueller-Hinton Agar
Incubation Conditions35–37°C for 16–20 hours
Storage-20°C (long-term); 2–8°C (short-term)
Shelf LifeUntil expiration date on packaging
Pack Sizes10, 30, 50, or 100 strips per pack

Quality Control Data

Control OrganismExpected MIC Range (μg/mL)
Escherichia coli ATCC® 259220.03–0.12
Pseudomonas aeruginosa ATCC® 278531–4
Klebsiella pneumoniae ATCC® BAA-1705 (KPC-positive)≥16
Acinetobacter baumannii ATCC® 19606≥8

Interpretation of Results

MIC ValueInterpretation
≤1 μg/mLSusceptible
2–4 μg/mLIntermediate
≥8 μg/mLResistant

Refer to CLSI/EUCAST guidelines for specific interpretation criteria.

Advantages

FeatureBenefit
Wide Concentration RangeDetects resistance across a broad spectrum.
High SensitivityAccurate identification of resistant isolates.
Ease of UseSimplifies MIC determination with clear visual results.
Standards ComplianceAdheres to CLSI and EUCAST testing protocols.

Comparison with Alternative MIC Testing Methods

MethodLiofilchem™ MTS™ ImipenemBroth MicrodilutionDisk Diffusion
Ease of UseEasyLabor-intensiveEasy but less precise
Time to Results16–20 hours16–20 hours16–20 hours
Quantitative MIC ValueYesYesNo
Resistance DetectionHigh sensitivityHigh sensitivityModerate sensitivity

Conclusion

The Liofilchem™ MTS™ Imipenem IMI 0.002-32 MIC Test Strips are a reliable and efficient solution for detecting imipenem resistance. Their precision, ease of use, and adherence to global standards make them indispensable for clinical laboratories, research institutions, and pharmaceutical companies monitoring antimicrobial resistance. 

  • Pack Size:   100/pk 10/pk 30/pk
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