Liofilchem™ MTS™ Ciprofloxacin CIP 0.002-32, MIC Test strips

The Liofilchem™ MTS™ Ciprofloxacin MIC Test Strips are advanced diagnostic tools designed to determine the Minimum Inhibitory Concentration (MIC) of ciprofloxacin for various bacterial pathogens. These strips are vital for guiding effective antimicrobial therapy, tracking resistance patterns, and supporting infection control measures in clinical microbiology laboratories.

Key Features

1. Precision MIC Determination:
   • Gradient range of 0.002–32 µg/mL, allowing precise quantification of bacterial susceptibility to ciprofloxacin.
   • Direct MIC reading from the point where the inhibition ellipse intersects the strip.
2. Regulatory Compliance:
   • FDA-cleared and CE-marked, ensuring conformity to global regulatory standards.
   • Compliant with ISO 11138, CLSI M100S, and other international guidelines.
3. Cost-Effective Alternative:
   • Offers comparable accuracy and reliability to other popular brands like Etest®, but at a lower cost.
4. Broad Compatibility:
   • Compatible with standard agar media and suitable for both routine diagnostics and resistance surveillance.
5. Ease of Use:
   • Simple application process with results obtainable in 16–20 hours under standard incubation conditions.
6. Quality Control:
   • Includes rigorous QC standards using reference strains:
     • E. coli ATCC® 25922 (MIC: 0.004–0.016 µg/mL)
     • P. aeruginosa ATCC® 27853 (MIC: 0.12–1 µg/mL).

Here’s a detailed tabular comparison of the Liofilchem™ MTS™ Ciprofloxacin MIC Test Strips with other popular MIC test strip brands like Etest® (bioMérieux):

Clinical Importance

Clinical Importance

• Therapeutic Guidance:
  • Supports the selection of appropriate ciprofloxacin dosages for treating bacterial infections.
• Resistance Monitoring:
  • Tracks resistance trends in critical pathogens like E. coli, P. aeruginosa, and Neisseria gonorrhoeae, which are increasingly exhibiting resistance to fluoroquinolones.
• Infection Control:
  • Helps prevent the spread of ciprofloxacin-resistant bacteria in healthcare settings.

Advantages Over Competitors

• Cost Efficiency: More affordable than Etest® strips without compromising performance.
• Global Availability: Widely distributed and supported for diverse clinical and research needs.
• Range of Products: Liofilchem™ offers a broad catalog of MIC test strips for various antimicrobials.

Applications

• Routine clinical microbiology for susceptibility testing.
• Surveillance of antibiotic resistance.
• Research on ciprofloxacin efficacy and resistance mechanisms.
• Validation of automated or manual susceptibility testing systems.

Quality Control Information:

To ensure the reliability and accuracy of the MTS™ Ciprofloxacin strips, it is imperative to perform quality control (QC) testing using standard reference strains. According to Liofilchem's guidelines, the following QC strains and their acceptable MIC ranges should be used:

These ranges are established based on the Clinical and Laboratory Standards Institute (CLSI) M100S Performance Standards for Antimicrobial Susceptibility Testing.

Procedure for Quality Control Testing:

1. Inoculum Preparation: Prepare a bacterial suspension of the QC strain in saline to match a 0.5 McFarland standard.
2. Inoculation: Using a sterile swab, evenly inoculate the entire surface of a Mueller-Hinton agar plate.
3. Application of MTS™ Strip: Place the MTS™ Ciprofloxacin strip onto the inoculated agar surface using sterile forceps or an applicator, ensuring full contact with the agar.
4. Incubation: Incubate the plate in an inverted position at 35 ± 2°C for 16–20 hours in an ambient atmosphere.
5. Reading Results: After incubation, read the MIC where the edge of the inhibition ellipse intersects the strip. The MIC should fall within the specified acceptable range for the QC strain.

Interpretation:

• If the MIC values for the QC strains fall within the acceptable ranges, the test is considered valid, and patient isolate results can be reported.
• If the MIC values are outside the acceptable ranges, investigate potential causes such as inoculum density, incubation conditions, or strip integrity before proceeding with patient isolate testing.

Adhering to these QC procedures ensures the accuracy and reliability of susceptibility testing using Liofilchem™ MTS™ Ciprofloxacin MIC Test Strips.

The Liofilchem™ MTS™ Ciprofloxacin MIC Test Strips are reliable, cost-effective, and easy-to-use diagnostic tools. They enable precise MIC determination for ciprofloxacin, helping clinicians and microbiologists optimize antimicrobial therapy and combat the growing challenge of antibiotic resistance. Their compliance with international standards and compatibility with common lab practices make them a preferred choice in clinical and research laboratories worldwide.