Liofilchem™ MTS™ Ceftriaxone CRO 0.016-256, MIC Test strips

The Liofilchem™ MTS™ Ceftriaxone (CRO) 0.016–256 μg/mL MIC Test Strips are specialized tools designed to determine the Minimum Inhibitory Concentration (MIC) of ceftriaxone against various microorganisms. These strips are integral in assessing microbial susceptibility, guiding effective antimicrobial therapy, and monitoring resistance patterns.

Product Overview:

• Antibiotic: Ceftriaxone (CRO)
• Concentration Range: 0.016 to 256 μg/mL
• Format: MIC Test Strips
• Purpose: Quantitative determination of ceftriaxone MIC

Test Procedure:

1. Inoculum Preparation:
   • Select well-isolated colonies from an overnight culture.
   • Suspend the colonies in sterile saline or broth to achieve a turbidity equivalent to a 0.5 McFarland standard.
2. Inoculation of Agar Plate:
   • Dip a sterile swab into the prepared inoculum suspension.
   • Remove excess liquid by pressing the swab against the tube wall.
   • Evenly streak the swab over the entire surface of a Mueller-Hinton agar plate to ensure a uniform lawn of growth.
3. Application of MTS™ Strip:
   • Allow the inoculated plate to dry for approximately 15 minutes at room temperature.
   • Using sterile forceps, place the MTS™ Ceftriaxone strip onto the agar surface with the concentration scale facing upward.
   • Ensure complete contact between the strip and the agar surface; do not reposition once applied.
4. Incubation:
   • Incubate the plate in an inverted position at 35 ± 2°C for 16–20 hours under ambient atmospheric conditions.
5. Reading and Interpretation:
   • After incubation, observe the plate for an elliptical zone of inhibition around the strip.
   • Determine the MIC by identifying the point on the scale where the edge of the inhibition ellipse intersects the strip.
   • Interpret the MIC value using established clinical breakpoints from CLSI or EUCAST guidelines.

Clinical Significance:

Ceftriaxone is a third-generation cephalosporin with a broad spectrum of activity against Gram-positive and Gram-negative bacteria. Determining the MIC of ceftriaxone is crucial for:

• Guiding Therapy: Ensuring appropriate dosing and selection of ceftriaxone for treating infections.
• Resistance Monitoring: Identifying resistant strains to inform treatment decisions and public health strategies.

Accurate MIC determination aids in optimizing patient outcomes and combating antimicrobial resistance.

Regulatory Data:

The Liofilchem™ MTS™ Ceftriaxone MIC Test Strips comply with international standards for antimicrobial susceptibility testing. They are designed to align with guidelines from:

• Clinical and Laboratory Standards Institute (CLSI): Provides standardized methods and interpretive criteria for antimicrobial susceptibility testing.
• European Committee on Antimicrobial Susceptibility Testing (EUCAST): Offers guidelines and breakpoints for interpreting MIC results.

Adherence to these standards ensures the reliability and accuracy of test results.

Comparison with Other Brands:

Several manufacturers offer MIC test strips for ceftriaxone, including bioMérieux's ETEST® and HiMedia's HiComb MIC Test Strips. Below is a comparative overview:

Advantages of Liofilchem™ MTS™ Ceftriaxone Strips:

• Material Quality: Made of high-quality paper, which may offer better adherence to the agar surface compared to plastic strips, potentially leading to more accurate results.
• Packaging Flexibility: Available in various pack sizes (10, 30, or 100 strips), allowing laboratories to choose quantities that best fit their testing frequency and budget.
• Cost-Effectiveness: Generally more affordable than some competitors, making them a cost-effective choice for routine laboratory use.
• Wide Range of Antimicrobials: Liofilchem offers a broad spectrum of MIC test strips for over 150 antimicrobial agents, providing comprehensive testing options.

In summary, Liofilchem™ MTS™ Ceftriaxone MIC Test Strips provide a reliable, flexible, and cost-effective solution for determining the MIC of ceftriaxone, with advantages in material quality and packaging options over some competitors.