Liofilchem® SensiQuattro Gram-negative

The SensiQuattro Gram-negative System is a diagnostic tool designed for the determination of the Minimum Inhibitory Concentration (MIC) of gram-negative bacteria using the broth microdilution method. It provides an efficient and accurate antibiogram evaluation, helping to establish microbial sensitivity to antibiotics in compliance with EUCAST or CLSI guidelines.

Key Features:

1. Comprehensive MIC Determination:
   • Enables evaluation of the MIC for 9 antibiotics in four two-fold dilutions and 1 antibiotic in three two-fold dilutions.
   • Supports antimicrobial susceptibility testing based on EUCAST and CLSI interpretative criteria.
2. User-Friendly Design:
   • 40-well plate containing pre-dried antibiotics.
   • Includes a growth control well for validation of microbial growth.
3. Wide Compatibility:
   • Suitable for gram-negative bacterial species including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella typhimurium.
4. Standardized Protocol:
   • Uses 0.5 McFarland Standard for microbial suspension preparation.
   • Incubation at 36±1°C for 18–24 hours ensures reproducible results.
5. Flexible Storage Conditions:
   • Stable at 2–8°C, with temporary tolerance for storage at higher temperatures during transport.

Kit Contents:

Antibiotic Configuration (40 Wells):

Principle of the Method:

The SensiQuattro Gram-negative system combines dried antibiotics, growth factors, and an indicator in each well. After rehydration with a standardized microbial suspension, the system undergoes incubation. Results are interpreted based on color changes in the wells:

• Yellow/Grey: Good microbial growth (positive growth control).
• Blue: Growth inhibited by the antibiotic.

MIC values are recorded as the lowest concentration of the antibiotic that inhibits visible growth.

Test Procedure:

1. Preparation:
   • Bring the plate to room temperature.
   • Prepare a 0.5 McFarland suspension using the physiological solution provided.
2. Inoculation:
   • Transfer 0.2 mL of suspension into the provided suspension medium.
   • Dispense 0.15 mL into each well using a multichannel pipette.
3. Incubation:
   • Cover the plate with the lid and incubate at 36±1°C for 18–24 hours.
4. Interpretation:
   • Observe well color changes to determine MIC.
   • Use EUCAST or CLSI criteria for antibiotic susceptibility interpretation.

Quality Control:

Each batch undergoes rigorous testing with the following reference strains:

• Escherichia coli ATCC® 25922
• Klebsiella pneumoniae ATCC® 700603
• Enterobacter cloacae ATCC® 13047
• Pseudomonas aeruginosa ATCC® 27853
• Salmonella typhimurium ATCC® 14028

Storage and Stability:

• Storage: 2–8°C in original packaging.
• Transport Stability: Tolerates 18–25°C for up to 4 days, or 35–39°C for 48 hours.
• Shelf Life: Valid until the expiration date on the label.

Applications:

1. Clinical Microbiology:
   • Determine susceptibility of gram-negative bacteria to antibiotics in clinical isolates.
2. Research and Development:
   • Study antimicrobial resistance patterns and test novel antibiotics.
3. Pharmaceutical Testing:
   • Validate the efficacy of antibiotics in development.
4. Epidemiological Studies:
   • Monitor resistance trends in gram-negative bacterial populations.

Ordering Information:

Related Products:

1. McFarland 0.5 Barium Sulfate Standard (Ref: 80400)
2. Multichannel Pipette (50–300 µL) (Ref: 96759)
3. Solution Reservoir for Multichannel Pipette (Ref: 96761)

Regulatory Notes:

• In Vitro Diagnostic (IVD): For laboratory use only.
• Safety: Follow aseptic and pathogen safety guidelines.

The SensiQuattro Gram-negative System provides a robust, standardized method for MIC determination, facilitating the accurate identification of antibiotic resistance in gram-negative bacteria. This easy-to-use system offers reliable results for both clinical and research applications.