Liofilchem® Integral System Streptococchi

The Liofilchem® Integral System Streptococchi is an advanced 24-well colorimetric testing system designed for the simultaneous identification and susceptibility testing of Streptococcal species. The system uses a combination of biochemical reactions and antibiotic susceptibility assays to deliver accurate results within 18-24 hours. This makes it a vital tool for clinical microbiology laboratories and food safety research facilities.

Key Features:

1. Comprehensive Biochemical Identification:
   • Identifies Streptococci through 9 unique biochemical tests, including:
     • Hydrolysis of PYR, esculin, and hippurate.
     • Fermentation of arabinose, mannitol, and raffinose.
     • Sensitivity to bacitracin and optochin.
2. Antibiotic Susceptibility Testing (AST):
   • Tests against 7 antibiotics, each at two concentrations:
     • Erythromycin, Co-Trimoxazole, Pefloxacin, Cephalothin, Teicoplanin, Rifampicin, Ampicillin-Sulbactam, and Piperacillin.
3. Numerical Code Identification:
   • Generates a three-digit numerical code based on the combination of positive and negative biochemical reactions for rapid species identification using a reference table or online software.
4. Rapid Turnaround Time:
   • Provides identification and susceptibility results within 18-24 hours after incubation.
5. Validated and Reliable:
   • Every batch is rigorously quality-checked against ATCC reference strains, ensuring high accuracy and consistency.

Specifications:

Kit Components:

Additional Items Required (Not Included):

• Physiological Solution (Ref. 20095)

Applications:

1. Clinical Microbiology:
   • Identify pathogenic Streptococcus species and determine their antibiotic susceptibility.
2. Food and Environmental Microbiology:
   • Detect and test Streptococci in food samples and environmental samples for safety compliance.
3. Research and Academia:
   • Conduct microbial studies related to Streptococcal infections and antimicrobial resistance.

How It Works:

1. Preparation of Bacterial Suspension:

• Ensure the microorganism is recently isolated (18-24 hours).
• Suspend one or more colonies from solid culture media in Physiological Solution (Suspension A).

2. System Inoculation:

• Biochemical Identification:
  • Add 0.2 mL of Suspension A into wells 1–7.
  • Place a PYR-Disc in well 1-PYR.
• Susceptibility Testing:
  • Add 50 µL of Suspension A into the Inoculum Broth (Suspension B).
  • Add 0.2 mL of Suspension B into wells 8–24.

3. Incubation:

• Cover the system and incubate at 36 ± 1°C for 18–24 hours.

4. Post-Incubation:

• Add reagents as follows:
  • PYR Reagent: Add 2 drops to well 1-PYR.
  • Ninhydrin Reagent: Add 2 drops to well 3-IPP.
• Wait 15-20 minutes, observe color changes, and interpret results.

Result Interpretation:

1. Biochemical Identification:
   • Positive and negative reactions in wells 1–9 generate a three-digit code for organism identification:
     • 1: First test positive in a group.
     • 2: Second test positive in a group.
     • 4: Third test positive in a group.
2. Susceptibility Testing:
   • Observe color changes in wells 10–24:
     • Red/Red: Sensitive.
     • Yellow/Red: Intermediate sensitivity.
     • Yellow/Yellow: Resistant.

Key Benefits:

1. Time-Saving:
   • Combines identification and antimicrobial susceptibility testing (AST) in a single system.
2. Comprehensive Testing:
   • Covers biochemical tests and antibiotics for thorough profiling of Streptococcus species.
3. Accurate Identification:
   • Utilizes a numerical code system for precise species identification.
4. Cost-Effective:
   • Reduces the need for separate tests, saving resources and labor.
5. Validated Results:
   • Consistent accuracy, validated against ATCC reference strains.

Antibiotics Tested:

• Erythromycin (ERY): 1 µg/mL, 4 µg/mL
• Co-Trimoxazole (SXT): 8 µg/mL
• Pefloxacin (PEF): 1 µg/mL, 4 µg/mL
• Cephalothin (KF): 8 µg/mL, 32 µg/mL
• Teicoplanin (TEC): 4 µg/mL, 16 µg/mL
• Rifampicin (RD): 4 µg/mL, 16 µg/mL
• Ampicillin-Sulbactam (AMP): 8-4 µg/mL, 16-8 µg/mL
• Piperacillin (PRL): 16 µg/mL, 64 µg/mL

Validation and Quality Control:

• Validated with ATCC reference strains:
  • Streptococcus pyogenes (ATCC® 19615)
  • Streptococcus pneumoniae (ATCC® 6303)
  • Enterococcus faecalis (ATCC® 19433)
  • Streptococcus agalactiae (ATCC® 13813)

Storage and Handling:

• Store at 2°C–8°C in its original packaging.
• Avoid heat and temperature fluctuations.
• Discard expired systems or those showing signs of deterioration.

References:

1. Ronald M. Atlas. Handbook of Microbiological Media, 4th Edition (2010).
2. Patrick R. Murray et al. Manual of Clinical Microbiology, 8th Edition (2003).
3. Edwin H. Lennette. Manual of Clinical Microbiology, 4th Edition (1985).

For Research Use Only:

This product is intended for research purposes only and is not approved for diagnostic or therapeutic use.