Thermo Scientific™ Invitrogen™ Ni-NTA Purification System with Antibody, with Anti-His (C-term)-HRP Antibody

The Invitrogen™ Ni-NTA Purification System with Antibody offers a comprehensive solution for the efficient isolation and detection of recombinant proteins containing a C-terminal or N-terminal polyhistidine tag (6xHis). This complete kit combines Ni-NTA nickel-chelating agarose resin with native and denaturing purification buffers, along with an HRP-conjugated Anti-His (C-terminal) antibody for downstream immunodetection, such as western blotting or ELISA.

This dual-functionality platform enables high-yield purification and subsequent visualization of His-tagged proteins from bacterial, yeast, or mammalian expression systems—making it ideal for molecular biology, proteomics, and protein expression research.

Key Features & Benefits

• Dual Purification Capability
  Includes buffer systems for both native and denaturing conditions to accommodate proteins of varying solubility.
• Highly Specific His-Tag Binding
  Uses Ni-NTA (nickel-nitrilotriacetic acid) agarose resin with high affinity and specificity for polyhistidine-tagged proteins.
• Immunodetection Ready
  Supplied with an Anti-His (C-terminal)-HRP conjugated antibody, facilitating direct detection after purification.
• Convenient Prepacked Columns
  Six prefilled 2 mL columns reduce handling time and variability.
• Flexible Application Range
  Supports downstream use in western blotting, ELISA, pull-down assays, and activity-based studies.

Kit Components

• Ni-NTA Agarose Resin: 10 mL (high-binding capacity)
• 2 mL Resin Columns: 6 columns
• Native & Denaturing Buffers: For condition-specific purification workflows
• Anti-His (C-term)-HRP Antibody: For immunodetection (western blot/ELISA)
• Comprehensive User Guide: For standardized protocols

Technical Specifications

Applications

• His-tagged protein purification
• Western blot validation of His-tag proteins
• Pull-down assays for protein–protein interactions
• Protein structural and functional studies
• Recombinant protein expression workflows

The Invitrogen™ Ni-NTA Purification System with Anti-His (C-term)-HRP Antibody delivers robust and reproducible protein purification with built-in detection flexibility. It is ideally suited for researchers seeking reliable, high-affinity isolation of His-tagged proteins under various expression and solubility conditions. Whether you're working with bacterial lysates or more complex systems, this kit streamlines your workflow from purification to analysis.