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Invitrogen™ PureLink™ Genomic Plant DNA Purification Kit, 50 preps
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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Efficient Isolation of High-Quality Genomic DNA from a Wide Variety of Plant Tissues
The Invitrogen™ PureLink™ Genomic Plant DNA Purification Kit provides a rapid, reliable method for isolating high-quality total DNA from diverse plant species in under 40 minutes. Designed for manual, spin column-based workflows, this kit is ideal for researchers working with challenging plant materials, including spinach, tomato, soy, wheat, Arabidopsis, corn, mushroom, and more.
Utilizing silica-based spin column technology, the protocol does not require organic solvents, ensuring a safe, clean, and efficient DNA purification workflow. The purified DNA is suitable for a wide range of downstream applications such as PCR, restriction enzyme digestion, hybridization, Southern blotting, and sequencing.
Key Features & Benefits
- Fast Protocol – Isolate total genomic DNA in less than 40 minutes
- High Purity and Yield – A260/A280 > 1.80; yields up to 14.6 µg from 100 mg of plant tissue
- Safe & Simple – Organic solvent-free protocol with spin column-based isolation
- Versatile Sample Compatibility – Works with a variety of fresh or frozen plant tissues
- Optimized Elution Format – Dual elution (2 × 100 µL) maximizes recovery (>90%)
- Includes RNase A – Reduces RNA contamination without additional handling
Applications
- PCR and qPCR
- Southern blotting
- Nucleic acid labeling and hybridization
- Restriction enzyme digestion
- DNA sequencing
- Plant genotyping and molecular diagnostics research
Compatible Sample Types
- Leaves – Tomato, spinach, wheat, Arabidopsis, alfalfa sprouts
- Sprouts and Roots – Soybean, sunflower, wheatgrass
- Fungi – Mushrooms
- Chloroplast-rich tissues
- Frozen or liquid nitrogen-treated samples
Technical Specifications
| Attribute | Specification |
|---|---|
| Catalog Number | K183001 |
| Format | Spin Column (Silica Membrane) |
| Sample Input | Up to 100 mg plant tissue |
| Elution Volume | 100–200 µL (2 × 100 µL recommended) |
| Yield | Up to 14.6 µg DNA per prep |
| Purity | A260/A280 ≥ 1.80 |
| Test Time | ~40 minutes |
| Final Product | Genomic DNA (gDNA) |
| For Use With | PCR, Southern blotting, sequencing, hybridization |
| Scale | Mini prep |
| High-throughput Compatible | No (manual use only) |
| Shipping Condition | Room temperature |
| Unit Size | 50 preps |
| Regulatory Use | For Research Use Only |
Kit Contents & Storage Conditions
| Component | Volume | Storage |
|---|---|---|
| Resuspension Buffer R2 | 12.5 mL | Room Temp |
| Precipitation Buffer N2 | 5 mL | Room Temp |
| Binding Buffer B4 | 7.5 mL | Room Temp |
| Wash Buffer W4 | 25 mL | Room Temp |
| Wash Buffer W5 | 10 mL | Room Temp |
| Elution Buffer | 10 mL | Room Temp |
| 20% SDS | 0.75 mL | Room Temp |
| RNase A | 0.75 mL | Room Temp |
| PureLink™ Spin Columns | 50 units | Room Temp |
| Collection Tubes (for elution) | 50 units | Room Temp |
The Invitrogen™ PureLink™ Genomic Plant DNA Purification Kit is an ideal choice for researchers who require high-yield, high-purity DNA from challenging plant tissues in a fast, user-friendly format. With its robust spin column technology, ready-to-use reagents, and compatibility with a wide range of plant species, this kit supports high-quality genomic DNA isolation for critical downstream applications.