Invitrogen™ UltraPure™ Glycerol

The Invitrogen™ UltraPure™ Glycerol is a high-purity, molecular biology-grade reagent designed for enzyme concentration, storage, and purification applications. With a purity of ≥99%, this redistilled glycerol is certified free of DNase, RNase, and protease activity, making it ideal for sensitive molecular biology workflows.

A 50% (v/v) glycerol solution remains liquid at -20°C, making it a preferred choice for enzyme stabilization and cryopreservation. Additionally, glycerol's high density (1.26 g/mL) makes it a key component in electrophoresis loading buffers and density gradient centrifugation.

Key Features & Benefits

1. Ultra-Pure, Molecular Biology Grade

• ≥99% purity (redistilled) ensures high reliability and minimal contamination.
• Free from DNase, RNase, and protease activity, making it suitable for RNA and DNA research.
• Strict quality control testing ensures batch-to-batch consistency.

2. Enzyme Stabilization & Storage

• Prevents enzyme degradation by acting as a cryoprotectant.
• Maintains protein and enzyme activity at low temperatures.
• 50% (v/v) solutions remain liquid at -20°C, preventing freezing-related damage.

3. Versatile Use in Molecular Biology & Biochemistry

• Used in glycerol gradients for protein, bacteriophage, and organelle purification.
• Provides increased sample density for electrophoresis loading buffers.
• Helps stabilize protein-protein and nucleic acid interactions.

Specifications

Applications

1. Enzyme & Protein Storage

• Used as a cryoprotectant for long-term storage of enzymes.
• Helps prevent freezing damage to proteins and nucleic acids.
• Stabilizes DNA and RNA polymerases, restriction enzymes, and antibodies.

2. Electrophoresis & Molecular Biology

• Increases sample density in electrophoresis loading buffers.
• Prevents sample diffusion in agarose and polyacrylamide gels.
• Used in protein and nucleic acid electrophoresis applications.

3. Glycerol Gradients for Purification

• Used in density gradient centrifugation for protein, bacteriophage, and organelle isolation.
• Helps separate macromolecular complexes and ribosomes.

4. Cryopreservation & Cell Culture

• Protects cells, enzymes, and nucleic acids from freezing damage.
• Commonly used for cryopreserving bacterial and yeast cultures.

Pack Sizes & Catalog Numbers

Storage & Handling

• Store at room temperature.
• Keep the container tightly closed to prevent contamination.
• Ensure sterile handling for molecular biology applications.

Summary

The Invitrogen™ UltraPure™ Glycerol is a high-purity, molecular biology-grade glycerol ideal for enzyme stabilization, electrophoresis, and molecular biology applications. Its RNase-, DNase-, and protease-free formulation ensures reliability for sensitive research.