Invitrogen™ Tris (1 M), pH 7.0, RNase-free

The Invitrogen™ Ambion™ 1 M Tris Buffer (pH 7.0, RNase-Free) is a high-purity, ready-to-use buffer designed for molecular biology applications. This RNase-free solution provides a stable buffering system suitable for RNA research, nucleic acid extraction, PCR, and electrophoresis buffers.

Tris buffers are widely used in molecular biology due to their consistent pH stability. The pH 7.0 formulation is particularly effective for RNA integrity preservation and enzyme reactions, ensuring optimal performance in RNA/DNA research and enzymatic assays.

Key Features & Benefits

1. High-Purity, RNase-Free Molecular Biology Grade

• Certified RNase-, DNase-, and protease-free, making it ideal for RNA and DNA research.
• Manufactured in nuclease-free facilities to prevent contamination.
• Rigorously tested for non-specific endonuclease and exonuclease activity.

2. Pre-Mixed, Ready-to-Use 1 M Tris Buffer (pH 7.0)

• Eliminates preparation time and ensures accuracy.
• Maintains pH stability, preventing degradation of nucleic acids and proteins.
• Economical and reliable for large-scale molecular biology applications.

3. Essential for Nucleic Acid & Protein Research

• Prevents RNA degradation in RNA isolation and purification workflows.
• Used in cDNA synthesis, RT-PCR, and hybridization reactions.
• Forms a core component in TE buffer (Tris-EDTA) for DNA/RNA storage and sequencing.

Specifications

Applications

1. Molecular Biology & RNA Applications

• Maintains RNA stability in RNA extraction and storage solutions.
• Used in Northern and Southern blot hybridization buffers.
• Provides pH stability in enzyme reactions involving RNA.

2. PCR, qPCR, and RT-PCR Buffer Systems

• Ensures optimal primer binding and extension in PCR-based reactions.
• Used in cDNA synthesis and RNA sequencing workflows.
• Maintains consistent pH for enzyme reactions.

3. Electrophoresis & Nucleic Acid Gel Buffers

• Used in TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) buffer systems.
• Ensures stable pH conditions for agarose and PAGE electrophoresis.
• Prevents nucleic acid degradation during long electrophoresis runs.

4. Protein Biochemistry & Enzyme Reactions

• Maintains pH stability in protein purification and Western blotting buffers.
• Used in restriction digestion, ligation, and DNA polymerase reactions.

Pack Sizes & Catalog Numbers

Storage & Handling

• Store at room temperature for long-term stability.
• Keep the container tightly closed to prevent contamination.
• Use sterile pipetting techniques to maintain RNase-free conditions.

The Invitrogen™ Ambion™ 1 M Tris Buffer, pH 7.0 is a high-purity, pre-mixed, RNase-free buffer ideal for RNA research, PCR, electrophoresis, and enzyme reactions. Its ready-to-use formulation ensures accurate pH control and contamination-free use, making it a critical reagent for molecular biology applications.