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Invitrogen™ Mouse IgG (Total) Uncoated ELISA Kit with Plates
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Quantitative measurement of total mouse IgG levels in serum, plasma, or culture supernatants
The Invitrogen™ Mouse IgG (Total) Uncoated ELISA Kit with Plates is a high-sensitivity, plate-based immunoassay designed to quantitatively detect total mouse IgG in biological fluids. The kit includes pre-matched antibody pairs, a high-affinity ELISA plate, and essential reagents to generate accurate and reproducible standard curves and sample measurements.
This uncoated ELISA format allows for greater flexibility and customization in coating conditions while providing reliable detection in serum, plasma, and culture supernatants. The kit is ideal for applications such as immune response studies, antibody production monitoring, and pharmacokinetics.
Key Features
- Quantitative Detection: Measures total IgG concentration in mouse samples with a detection range of 1.56–100 ng/mL
- High Sensitivity: Analytical sensitivity as low as 1.56 ng/mL
- Low Sample Volume: Requires only 10 µL of serum, plasma, or supernatant
- Flexible Format: Uncoated kit includes 96-well plates; wash buffer and stop solution sold separately
- Convenient Workflow: Hands-on time of 1 hour 45 minutes; total assay time ~24 hours
- Validated for Mouse IgG (All Isotypes)
Applications
- Quantification of total IgG in mouse serum and plasma
- Monitoring antibody production in hybridoma or recombinant systems
- Evaluation of immune responses in mouse models
- Quality control for antibody-based formulations
- Pharmacokinetics and immunogenicity studies
Kit Contents
| Component | Description |
|---|---|
| Capture Antibody | Pre-titrated, purified mouse IgG-specific antibody |
| Detection Antibody | Pre-titrated, HRP-conjugated anti-mouse IgG |
| Standard | Recombinant mouse IgG protein |
| 10X Coating Buffer | For capture antibody coating |
| 5X ELISA/ELISPOT Diluent | For blocking and dilution of detection reagents |
| Substrate | 1X TMB substrate for HRP signal detection |
| 96-Well Plate | High-affinity plate (included in SKUs ending in -22, -76, -86) |
| Certificate of Analysis | Lot-specific information and dilution instructions |
Note: Wash buffer and stop solution are not included and must be purchased separately.
Technical Specifications
| Parameter | Value |
|---|---|
| Catalog Numbers | 88-50400-22, 88-50400-76, 88-50400-86 |
| Assay Format | Uncoated ELISA with included plate |
| Target | Mouse Immunoglobulin G (Total IgG) |
| Species Reactivity | Mouse |
| Sample Types | Plasma, Serum, Culture Supernatants |
| Sample Volume Required | 10 µL |
| Analytical Sensitivity | 1.56 ng/mL |
| Assay Range | 1.56–100 ng/mL |
| Readout | Absorbance (TMB substrate) |
| Detection Method | HRP colorimetric |
| Instrument Required | Standard Microplate Reader (450 nm) |
| Hands-on Time | ~1 hour 45 minutes |
| Time to Results | ~24 hours 30 minutes |
| Storage Conditions | 2–8°C |
| Shipping Conditions | Shipped on wet ice |
| Regulatory Status | Research Use Only (RUO) |
Ordering Information
| Catalog Number | Pack Size | Plates Included |
|---|---|---|
| 88-50400-22 | 2 × 96 tests | Yes |
| 88-50400-86 | 10 × 96 tests | Yes |
| 88-50400-76 | 20 × 96 tests | Yes |
The Invitrogen™ Mouse IgG (Total) Uncoated ELISA Kit with Plates provides a sensitive and customizable platform for accurately quantifying total IgG in a variety of mouse-derived samples. Its flexible format and high specificity make it a reliable choice for immunology, vaccine development, and antibody research in mouse models.
- Pack Size: 2 x 96 Tests Plate 10 x 96 Tests Plate 20 x 96 Tests Plate