Invitrogen™ Low Fluorescence PVDF Membranes

Invitrogen™ Low Fluorescence PVDF Membranes are specifically engineered for fluorescent Western blotting, total protein normalization, and dot blot applications, providing ultra-low autofluorescence, high binding capacity, and excellent reproducibility. With reduced background signal across a broad spectrum (280–800 nm), these membranes enable enhanced signal-to-noise ratios, ideal for multiplex fluorescent detection.

With a uniform 0.3 µm pore structure, these membranes support consistent protein retention and transfer across a wide molecular weight range. They are compatible with both wet tank and semi-dry transfer systems, and are available in a range of sheets, rolls, and pre-assembled sandwich formats to suit any lab workflow.

Key Features and Benefits

• Ultra-low autofluorescence – ideal for IR, NIR, and RGB fluorescent detection
• High protein binding – ensures sensitive detection of both low- and high-abundance targets
• Improved signal-to-noise ratio – enhances data quality and accuracy
• Consistent pore distribution (0.3 µm) – facilitates reproducible performance across all protein sizes
• Multiplex-ready – compatible with a wide range of fluorophores and chemiluminescent substrates
• Flexible formats – sheets, rolls, and membrane/filter paper sandwiches available
• Compatible with standard Western blotting workflows – no protocol changes required

Applications

• Fluorescent Western blotting (IR, near-IR, RGB dyes)
• Chemiluminescent protein detection
• Quantitative Western blotting and total protein normalization
• Dot blot assays and multiplex protein detection

Available Formats and Ordering Information

Storage & Handling

• Store flat at room temperature
• Avoid direct sunlight or exposure to chemical vapors
• Supplied dry—requires methanol activation prior to use

Invitrogen™ Low Fluorescence PVDF Membranes are designed for researchers who demand precision, sensitivity, and reproducibility in fluorescent and chemiluminescent protein detection. Whether you’re performing single-color quantification or complex multiplex assays, these membranes help deliver clean, consistent Western blot data with reduced background interference.