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Invitrogen™ LIVE/DEAD™ BacLight™ Bacterial Viability Kit
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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Two-Color Fluorescence Assay for Rapid Differentiation of Live and Dead Bacteria
Invitrogen™ LIVE/DEAD™ BacLight™ Bacterial Viability Kits provide a rapid and reliable method to assess bacterial viability based on membrane integrity. Utilizing a two-color fluorescence staining system with SYTO™ 9 (green) and propidium iodide (red), these kits allow easy differentiation between live (intact membranes) and dead or damaged (compromised membranes) cells within minutes.
Designed for use with fluorescence microscopy, flow cytometry, microplate assays, and other fluorescence-based instruments, these kits are ideal for researchers working with a wide range of bacterial species and mixed microbial populations.
Key Features and Benefits
Two-Color Membrane Integrity Assay
- SYTO™ 9 labels all bacteria (green), while propidium iodide (PI) penetrates only damaged cells (red), providing a clear visual or quantitative distinction between viable and non-viable cells.
Flexible Formats for Various Applications
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Choose from three kits depending on your workflow needs:
- L7007 – Premixed dye solutions for microscopy
- L7012 – Separate dyes for microscopy and quantitative assays
- L13152 – Premeasured, solvent-free dye pairs in sealed pipets for maximum convenience
Broad Compatibility
- Compatible with a wide range of bacterial genera, including E. coli, P. aeruginosa, S. aureus, B. subtilis, and more.
- Suitable for log-phase cultures, mixed species samples, and various physiological conditions.
Multipurpose Use
- Perform live/dead analysis via microscopy, flow cytometry, or microplate reader.
- Reagents can be diluted in common physiological buffers (e.g., PBS, pH 7.0–8.0).
- Each kit includes BacLight mounting oil for immobilized bacterial samples.
Applications
- Rapid bacterial viability assessment
- Antimicrobial susceptibility testing
- Biofilm viability and quantification
- Environmental and industrial microbiology
- Mixed culture live/dead cell differentiation
- Microbial contamination testing
Technical Specifications
| Property | Details |
|---|---|
| Catalog Numbers | L7007, L7012, L13152 |
| Assay Type | Fluorescence-based membrane integrity assay |
| Dyes | SYTO™ 9 (green), Propidium Iodide (red) |
| Excitation/Emission | 480/500 nm (green), 490/635 nm (red) |
| Cell Type | Bacteria |
| Detection Method | Fluorescence |
| Compatible Equipment | Fluorescence Microscope, Microplate Reader, Flow Cytometer |
| Format | Tubes, Slides, 96-well plates |
| Storage Conditions | –5°C to –30°C; protect from light |
| Shipping Condition | Room temperature |
| Product Line | BacLight™, LIVE/DEAD™ |
| Unit Size | Each (1 Kit) |
Kit Comparison
| Catalog Number | Format | Reagent Composition | Recommended Use |
|---|---|---|---|
| L7007 | Microscopy | Premixed SYTO 9 & PI in DMSO (2 formulations) | Fluorescence microscopy |
| L7012 | Microscopy & Quantitative Assays | Separate SYTO 9 (3.34 mM) and PI (20 mM) in DMSO | Microscopy, flow cytometry, microplates |
| L13152 | Viability Assay | 10 dry dye pairs in sealed pipets, reconstituted before use | Fieldwork, stability, convenience |
LIVE/DEAD™ BacLight™ Bacterial Viability Kits provide a trusted, flexible, and validated solution for assessing bacterial cell health. Whether using fluorescence microscopy, flow cytometry, or quantitative plate assays, these kits offer robust and reproducible results across a variety of bacterial strains and experimental conditions. Their simplicity, speed, and two-color readout make them indispensable tools for microbial research, diagnostics development, and antimicrobial efficacy studies.
- Application: Microscopy Viability Assay Microscopy, Quantitative Assays