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Invitrogen™ Lipofectamine™ 2000 Transfection Reagent
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Specifications:
| Application | Transfection | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-efficiency, serum-compatible lipid-based reagent for gene expression and gene silencing
The Invitrogen™ Lipofectamine™ 2000 Transfection Reagent is a gold-standard, lipid-based reagent used worldwide for efficient delivery of plasmid DNA, siRNA, and shRNA into a wide variety of adherent and suspension mammalian cells. Known for its broad applicability, high reproducibility, and low cytotoxicity, Lipofectamine™ 2000 is ideal for gene expression, gene knockdown, and co-transfection applications.
With compatibility across a wide range of cell types, and proven performance in the presence of serum, this reagent simplifies workflows and supports high-throughput screening platforms, stable cell line development, and robust protein expression.
Key Features
- High Transfection Efficiency: Achieve high expression levels in both common and difficult-to-transfect cell lines
- Co-Transfection Capable: Delivers siRNA and plasmid DNA simultaneously for efficient gene knockdown and expression
- Serum-Compatible: No media change required; transfection can proceed in complete growth medium
- High-Throughput Ready: Streamlined protocol supports automation and 96-well formats
- Stable Cell Line Generation: Reliable for creating stable transfectants in both adherent and suspension cells
- Easy-to-Use Protocol: Simple complex formation and single-step addition to cultures
- Sustainable Packaging: Aligned with green lab initiatives
Applications
- Transient gene expression in mammalian cells
- RNA interference (RNAi) using siRNA or shRNA
- Co-transfection for gene function studies
- Stable cell line establishment
- Reporter gene assays and high-throughput screening
Technical Specifications
| Specification | Details |
|---|---|
| Product Line | Lipofectamine™ |
| Transfection Technique | Lipid-based |
| Sample Types | Plasmid DNA, siRNA, shRNA, miRNA |
| Cell Types | Established cell lines, stem cells, primary cells |
| Serum Compatibility | Yes |
| High-throughput Compatible | Yes |
| Format Compatibility | 6-, 12-, 24-, 48-, 96-well plates; flasks |
| Shipping Conditions | Room temperature or wet ice |
| Storage Conditions | Store at 2–8°C. Do not freeze |
| Unit Size | Each |
Ordering Information
| Catalog Number | Pack Size |
|---|---|
| 11668030 | 0.3 mL |
| 11668027 | 0.75 mL |
| 11668019 | 1.5 mL |
| 11668500 | 15 mL |
Lipofectamine™ 2000 vs. Lipofectamine™ 3000
| Feature | Lipofectamine™ 2000 | Lipofectamine™ 3000 |
|---|---|---|
| Transfection Efficiency | High efficiency in most common cell lines | Superior efficiency, especially in hard-to-transfect cells |
| Cell Viability | Moderate; some toxicity depending on dose | Improved cell viability with lower toxicity |
| DNA Compatibility | Excellent | Excellent |
| RNA Compatibility | siRNA, shRNA | siRNA, mRNA, miRNA, shRNA |
| Co-Transfection (DNA + RNA) | Very good | Superior performance |
| CRISPR / Genome Editing Use | Limited application | Optimized for genome editing tools (CRISPR/Cas9, TALENs) |
| Transfection Range | Effective in many cell lines | Broadest cell compatibility (including stem and primary cells) |
| Serum Compatibility | Yes; no media change needed | Yes |
| High-throughput Compatibility | Yes | Yes |
| Enhancer Reagent Included | No | Yes (P3000™ Enhancer) |
| Ease of Use | Simple 2-component protocol | Simple, slightly more optimized with enhancer step |
| Scalability | Suitable for most culture formats | Highly scalable, from microplate to flask formats |
| Packaging Sustainability | Yes | Yes |
| Recommended For | Gene expression, RNAi, stable line creation | DNA/RNA co-transfection, genome editing, hard-to-transfect cells |
Invitrogen™ Lipofectamine™ 2000 remains a trusted, versatile transfection reagent offering unmatched efficiency, ease-of-use, and broad applicability for gene delivery workflows. Ideal for gene expression, knockdown studies, and high-throughput applications, it supports both manual and automated transfection processes, helping researchers achieve consistent and impactful results across cell types and research disciplines.
- Pack Size: 15 mL 0.75 mL 1.5 mL 0.3 mL