Invitrogen™ eBioscience™ IL-17A Monoclonal Antibody (eBio64DEC17), Brilliant Violet™ 786

The Invitrogen™ IL-17A Monoclonal Antibody (eBio64DEC17), conjugated to Brilliant Violet™ 786 (BV786), is a high-performance reagent for detecting human IL-17A. IL-17A is a pro-inflammatory cytokine secreted by Th17 cells, playing a pivotal role in autoimmune diseases and inflammation. This antibody is validated for flow cytometry and provides bright fluorescence for intracellular cytokine staining in immune cells.

Key Features:

1. Target and Specificity:
   • Recognizes human IL-17A, a cytokine involved in promoting inflammatory and immune regulatory responses.
   • Ideal for intracellular detection in CD4+ T cells and Th17 cells.
2. Clone and Conjugate:
   • Clone: eBio64DEC17, a reliable and reproducible clone for IL-17A detection.
   • Conjugate: Brilliant Violet™ 786, a tandem dye offering bright fluorescence with excitation at 407 nm and emission at 786 nm.
3. Validated Applications:
   • Optimized for flow cytometry, enabling robust analysis of IL-17A expression in human immune cells.
4. Bright and Stable Fluorescence:
   • BV786 provides high signal intensity with minimal background, making it ideal for multicolor flow cytometry panels.
5. Ready-to-Use:
   • Pre-titrated and easy to use, requiring 5 µL (0.5 µg) per test.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect intracellular IL-17A in human Th17-polarized CD4+ T cells.
   • Analyze immune cell cytokine production in multicolor flow panels.
2. Th17 Research:
   • Study IL-17A production and its role in Th17 differentiation pathways regulated by IL-23.
3. Autoimmune Disease Studies:
   • Investigate IL-17A’s involvement in conditions such as rheumatoid arthritis, multiple sclerosis, and psoriasis.
4. Cytokine Profiling:
   • Identify and quantify IL-17A expression in inflammation and immune response research.
5. Immunotherapy Research:
   • Explore IL-17A-related mechanisms in cancer and therapeutic interventions.

Key Benefits:

1. Bright Fluorescence:
   • BV786 offers superior brightness for clear identification of IL-17A-positive cell populations in flow cytometry.
2. Minimal Background:
   • High signal-to-noise ratio ensures reliable results, even in complex staining panels.
3. Reproducibility:
   • Pre-titrated and validated for consistent performance across experiments.
4. Optimized for Multicolor Panels:
   • BV786’s spectral properties make it highly compatible with other fluorochromes in multicolor panels.
5. Convenience:
   • Ready-to-use liquid formulation simplifies experimental workflows.

Usage Recommendations:

1. Sample Preparation:
   • Stimulate human peripheral blood CD4+ T cells with IL-23 or a similar reagent under Th17-polarizing conditions.
2. Staining Protocol:
   • Use the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) for optimal intracellular staining.
   • Add 5 µL (0.5 µg) of antibody per 100 µL of cell suspension.
3. Flow Cytometry:
   • Analyze samples using a flow cytometer equipped with a violet laser (407 nm).
4. Fixation:
   • Fix samples in IC Fixation Buffer to preserve fluorescence for up to 3 days.
5. Minimize Non-Specific Binding:
   • Use Brilliant Stain Buffer™ to reduce polymer dye interactions in complex staining panels.

Storage and Handling:

• Store at 4°C in the dark to protect the Brilliant Violet™ 786 fluorochrome.
• Avoid freezing, as freezing may degrade the antibody or fluorochrome.

Target Information:

• IL-17A:
  • A pro-inflammatory cytokine secreted by Th17 cells.
  • Plays a critical role in autoimmune diseases, inflammation, and host defense.
  • Stimulates epithelial and fibroblast cells to produce cytokines like IL-6, IL-8, and G-CSF, amplifying inflammatory responses.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.