Invitrogen™ eBioscience™ IFN gamma Monoclonal Antibody (4S.B3), PE

The Invitrogen™ IFN gamma Monoclonal Antibody (4S.B3), conjugated to PE (Phycoerythrin), is a high-quality reagent designed for the detection of human IFN gamma in intracellular and flow cytometry assays. IFN gamma, a key cytokine produced by activated T cells and NK cells, plays a vital role in immune regulation, macrophage activation, and antiviral activity. This antibody is optimized for intracellular staining, providing bright fluorescence and reproducible results for multicolor flow cytometry panels.

Key Features:

1. Target and Specificity:
   • Recognizes human IFN gamma, a cytokine involved in immune regulation, tumor suppression, and macrophage activation.
   • High specificity ensures accurate intracellular detection in immune cells.
2. Clone and Conjugate:
   • Clone: 4S.B3, a validated monoclonal antibody with consistent performance.
   • Conjugate: PE (Phycoerythrin), offering bright fluorescence with excitation at 565 nm and emission at 576 nm.
3. Validated Applications:
   • Optimized for intracellular staining and flow cytometry to study IFN gamma expression in human immune cells.
4. Bright and Stable Fluorescence:
   • PE provides high-intensity signals, minimizing background noise for clear identification of IFN gamma-positive cells.
5. Advanced Verification:
   • Verified using cell treatment to ensure the antibody binds specifically to its target antigen.
6. Ready-to-Use:
   • Supplied in liquid form with a concentration of 0.2 mg/mL, requiring only 0.25 µg per test.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect intracellular IFN gamma expression in activated T cells, NK cells, and other immune cells.
2. Th1 Research:
   • Study the role of IFN gamma in Th1 differentiation and immune responses.
3. Cytokine Profiling:
   • Analyze IFN gamma production in response to antigen or mitogen stimulation.
4. Immunotherapy Studies:
   • Explore the effects of IFN gamma in tumor immunity and therapeutic interventions.
5. Multicolor Staining Panels:
   • Compatible with complex staining protocols in immune research.

Key Benefits:

1. Bright Fluorescence:
   • PE ensures superior brightness, making it ideal for detecting low-abundance targets like IFN gamma.
2. Minimal Background:
   • High specificity of the 4S.B3 clone ensures accurate results with minimal non-specific binding.
3. Reproducibility:
   • Validated and pre-titrated for consistent performance across experiments.
4. Advanced Verification:
   • Verified for specificity using cell treatment, ensuring robust and reliable results.
5. User-Friendly:
   • Ready-to-use liquid formulation simplifies experimental workflows.

Usage Recommendations:

1. Sample Preparation:
   • Stimulate human peripheral blood mononuclear cells (PBMCs) or other immune cells with antigens or mitogens to induce IFN gamma production.
2. Staining Protocol:
   • Use the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) for optimal intracellular staining.
   • Add 0.25 µg of antibody per 100 µL of cell suspension.
3. Flow Cytometry:
   • Analyze using a flow cytometer equipped with a blue laser (488 nm) or yellow-green laser (561 nm).
4. Controls:
   • Include isotype controls (e.g., Mouse IgG1 kappa Isotype Control, PE) to assess non-specific binding.

Storage and Handling:

• Store at 4°C in the dark to protect the PE fluorochrome.
• Avoid freezing, as freezing may degrade the antibody or fluorochrome.

Target Information:

• IFN gamma:
  • A dimeric cytokine (~45 kDa) produced by activated T cells and NK cells.
  • Plays a critical role in immune responses, macrophage activation, and MHC expression.
  • Acts via the JAK-STAT signaling pathway and synergizes with other cytokines for immune regulation.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.