Human Hepatitis D Virus Antigen (HDV-Ag) ELISA Kit

The Human Hepatitis D Virus Antigen (HDV-Ag) ELISA Kit is a highly sensitive and specific assay designed for the qualitative and quantitative detection of HDV-Ag in human serum, plasma, culture media, and other biological fluids. This kit is a valuable tool for studying hepatitis D virus (HDV) infections, immune responses, and the interaction between HDV and hepatitis B virus (HBV).

Purpose

• Detection of HDV-Ag:
  • Identify and measure HDV antigens in human samples to support research on HDV infection and co-infection with HBV.
• Applications:
  • Suitable for human serum, plasma, and biological fluids in clinical research and diagnostic studies.

Principle

This ELISA kit utilizes a Sandwich ELISA technique to ensure specificity and accuracy in detecting HDV antigens. The process involves:

1. Pre-Coated Plate:
   • Microplate wells are pre-coated with an antibody specific to HDV-Ag.
2. Sample and Detection Antibody Addition:
   • Standards or test samples are added to the wells, where HDV-Ag in the sample binds to the immobilized antibody.
   • A biotin-conjugated detection antibody specific to HDV-Ag is added.
3. HRP-Streptavidin Conjugation:
   • Horseradish Peroxidase (HRP)-conjugated streptavidin is introduced, which binds to the biotin-labeled detection antibody.
4. Washing Step:
   • Unbound materials are washed away with wash buffer, minimizing background noise.
5. Substrate Reaction:
   • TMB substrate solution is added, which reacts with HRP to produce a blue product that changes to yellow after adding a stop solution.
6. Measurement:
   • Optical density (OD) is measured at 450 nm using a spectrophotometer. OD values are proportional to the HDV-Ag concentration.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects HDV antigens with minimal cross-reactivity.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, and other biological fluids.
3. Quantitative and Qualitative:
   • Measures HDV-Ag levels with high precision using a standard curve or detects its presence qualitatively.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a straightforward workflow.
5. Reproducibility:
   • Ensures consistent results across multiple experiments.

Kit Components

• Pre-coated Microplate with HDV-Ag capture antibody.
• Biotin-conjugated HDV detection antibody.
• HRP-Streptavidin conjugate.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Standards for generating the standard curve.
• Instruction manual.

Applications

• Hepatitis D Virus Research:
  • Study the epidemiology and pathogenesis of HDV infections.
• Co-Infection Studies:
  • Investigate the interaction between HDV and HBV in co-infected individuals.
• Therapeutic Development:
  • Evaluate drugs and therapeutic agents targeting HDV replication or antigen expression.
• Vaccine Development:
  • Assess immune responses to HDV antigens in vaccine trials.
• Clinical Research:
  • Support the diagnosis and monitoring of HDV-associated diseases.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of HDV-Ag.
2. Convenience:
   • Ready-to-use reagents simplify assay setup and execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Offers consistent results for robust data interpretation.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent stability.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Hepatitis D Virus Antigen (HDV-Ag) ELISA Kit is an essential tool for detecting and quantifying HDV antigens in biological samples. Its high sensitivity, specificity, and versatility make it indispensable for HDV research, co-infection studies with HBV, and therapeutic and vaccine development.