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Human Hemoglobin C (HbC) ELISA Kit
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Assay range: 2.6ng/ml -150ng/ml
Sensitivity: 0.5ng/ml
Standard: 180 ng/ml
Sample Volume: 10ul
Wavelength: 450nm
Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Grade | Molecular Biology | ||
The Human HbC ELISA Kit is a high-sensitivity, sandwich-based enzyme-linked immunosorbent assay (ELISA) designed for the quantitative detection of Hemoglobin C (HbC) in human serum, plasma, cell culture supernatants, tissue homogenates, and other biological fluids. This kit is particularly useful for research into hemoglobinopathies, erythrocyte disorders, and related clinical biomarkers in hematologic and genetic studies.
Key Features
- Assay Format: Sandwich ELISA
- Detection Range: 2.6 – 150 ng/mL
- Sensitivity: 0.5 ng/mL
- Sample Volume: 10 μL
- Assay Duration: Approx. 2.5 hours
- Storage Conditions: 2–8 °C
- Shelf Life: 6 months
-
Precision:
- Intra-assay CV <10%
- Inter-assay CV <12%
Assay Principle
This kit uses a double-antibody sandwich method. The microplate is pre-coated with a monoclonal antibody specific to Human HbC. After sample addition, HRP-conjugated anti-HbC antibodies bind to the captured antigen. A TMB substrate reacts with HRP to produce a blue color, which turns yellow upon addition of the stop solution. The absorbance is read at 450 nm, and the concentration is determined using a standard curve.
Kit Components
| Component | Volume / Quantity | Storage |
|---|---|---|
| Pre-coated Strip Plate | 96 wells | 2–8 °C |
| HbC Standard (180 ng/mL) | 0.5 mL × 1 vial | 2–8 °C |
| Standard Diluent | 1.5 mL × 1 vial | 2–8 °C |
| Sample Diluent | 6 mL × 1 vial | 2–8 °C |
| HRP-Conjugate Reagent | 6 mL × 1 vial | 2–8 °C |
| Chromogen Solution A | 6 mL × 1 vial | 2–8 °C |
| Chromogen Solution B | 6 mL × 1 vial | 2–8 °C |
| Stop Solution | 6 mL × 1 vial | 2–8 °C |
| Wash Buffer (30×) | 20 mL × 1 bottle | 2–8 °C |
| User Manual | 1 copy | Room Temp |
| Closure Plate Membranes | 2 sheets | Room Temp |
| Sealed Bag | 1 unit | Room Temp |
Validated Sample Types
- Serum / Plasma (EDTA, citrate-treated)
- Urine / Cerebrospinal Fluid / Pleural Fluid
- Cell Culture Supernatant
- Tissue Homogenate
Assay Procedure Overview
- Prepare standard dilutions (180 → 10 ng/mL).
- Add 40 μL sample diluent + 10 μL sample to each well.
- Incubate 30 minutes at 37 °C, sealed.
- Wash 5× using diluted wash buffer.
- Add 50 μL HRP-conjugate, incubate again.
- Wash and add 50 μL each of Chromogen A & B. Incubate in dark for 15 minutes.
- Add 50 μL stop solution. Measure absorbance at 450 nm within 15 minutes.
Performance Metrics
| Parameter | Specification |
|---|---|
| Assay Range | 2.6 – 150 ng/mL |
| Sensitivity | 0.5 ng/mL |
| Intra-Assay Precision | CV <10% |
| Inter-Assay Precision | CV <12% |
Standard curves are generated using log-log plotting. Final concentrations are calculated by applying the sample dilution factor.
The Human Hemoglobin C (HbC) ELISA Kit provides a reliable, specific, and sensitive method for detecting and quantifying HbC in a range of biological samples. With a broad dynamic range, low detection limit, and rigorous precision, this kit is a valuable tool for hematological research, especially in studies of hemoglobin variants and genetic blood disorders. The simple protocol and ready-to-use reagents make it ideal for researchers in both basic and clinical science.
- Pack Size: For 48 Tests For 96 Tests