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Sigma-Aldrich Silver Stain (Modified GMS) Kit
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Biological Stain | Forms | Liquid |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Microbiology grade | ||
The Silver Stain (Modified GMS) Kit, also known as the Modified Grocott Methenamine Silver (GMS) Fungus Stain, is designed for histologic demonstration of fungi, basement membranes, and some opportunistic organisms in human tissue specimens. This in vitro diagnostic tool is a manual, qualitative procedure that assists in the diagnosis of fungal infections when combined with other diagnostic information.
Key Features:
- Specialized Staining for Fungi:
- Highlights fungi, basement membranes, and some opportunistic organisms in tissue samples.
- Diagnostic Implications:
- Provides valuable data when combined with other diagnostic tests for fungal infections.
- Validated for In Vitro Use:
- Designed specifically for professional diagnostic use in clinical histology labs.
- Comprehensive Kit Components:
- Contains all necessary reagents for performing the staining procedure.
Kit Components:
| Product Number | Description | Volume |
|---|---|---|
| HT100-2 | Borax Solution | 100 mL |
| HT100-4 | Gold Chloride Solution | 100 mL |
| HT100-5 | Sodium Thiosulfate Solution | 100 mL |
| HT100-3 | Silver Methenamine Reagent (12 vials) | - |
| - | Periodic Acid Solution (Kit Only) | 100 mL |
Applications:
- Histologic Demonstration:
- Stains fungi, basement membranes, and opportunistic organisms in human tissue specimens.
- Fungal Infection Diagnostics:
- Aids in diagnosing fungal infections such as those caused by Candida, Aspergillus, and Cryptococcus.
- Basement Membrane Visualization:
- Highlights basement membranes in tissue sections for diagnostic evaluation.
Advantages:
- High Specificity:
- Accurately identifies fungal elements, providing clear and reproducible results.
- Comprehensive Kit:
- Includes all reagents required for the procedure, minimizing preparation time.
- Validated for Clinical Use:
- Designed for histopathology laboratories conducting diagnostic testing.
- Diagnostic Support:
- Provides valuable data to support clinical findings when combined with other diagnostic tests.
Procedure Overview:
- Prepare Tissue Sections:
- Use paraffin-embedded tissue sections mounted on slides.
- Staining Steps:
- Treat sections with periodic acid solution to oxidize fungal carbohydrates.
- Apply silver methenamine reagent to stain fungal elements and basement membranes.
- Tone the sections with gold chloride solution for enhanced visualization.
- Treat with sodium thiosulfate solution to remove unreacted silver.
- Counterstaining (Optional):
- Counterstain with light green or another compatible stain to enhance contrast.
- Microscopic Examination:
- Examine slides under a microscope to identify stained fungal elements.
Ordering Information:
| Catalog Number | Description | Pack Size |
|---|---|---|
| HT100A-1KT | Silver Stain (Modified GMS) Kit | 1 kit |
Storage and Handling:
- Store reagents at room temperature unless otherwise specified on the product label.
- Follow the expiration date indicated on the kit for optimal performance.
Related Products:
- Hematoxylin Counterstain: For enhancing contrast in stained tissue sections.
- Trichrome Stain Kit: For visualizing connective tissues in histology.
The Silver Stain (Modified GMS) Kit is a reliable and comprehensive tool for identifying fungal elements and related structures in histology. Its proven performance, ease of use, and high specificity make it essential for diagnostic labs focused on histopathology and infectious disease diagnostics.