HOT FIREPol® Probe Universal qPCR Kit, 3 x 1mL vials

The HOT FIREPol® Probe Universal qPCR Kit is a high-performance, probe-based qPCR master mix optimized for DNA/LNA hydrolysis probes (e.g., TaqMan® assays). Designed to deliver high specificity, sensitivity, and reproducibility, it supports both singleplex and duplex reactions across a wide range of real-time PCR instruments.

With a unique buffer formulation, the mix efficiently amplifies AT-rich, regular, and GC-rich templates (up to 75% GC content). The inclusion of dUTP allows UNG treatment for contamination prevention, while the hot-start chemistry provides clean, reliable amplification.

Key Features & Benefits

• Universal Compatibility – Works with most qPCR platforms (except capillary systems).
• Versatile Performance – Optimized for both singleplex and duplex assays.
• Superior GC-Rich Template Coverage – Efficient amplification of sequences with up to 75% GC content.
• High Specificity & Sensitivity – Ensures reliable quantification in demanding applications.
• Contamination Control – Incorporates dUTP for UNG treatment (UNG not included).
• Benchtop Stability – Preassembled reactions remain stable for up to 48 hours at room temperature.
• Convenience – Internal passive reference dye (ROX-based) ensures cross-platform usability.

Applications

• Probe-based DNA/LNA hydrolysis assays
• Quantification of DNA and cDNA targets
• Gene expression profiling
• Microbial detection
• Viral load determination

Kit Components

• HOT FIREPol® DNA Polymerase – Chemically modified hot-start enzyme
• 5× Probe Universal qPCR buffer (15 mM MgCl₂; final 3 mM in 1× reaction)
• dNTPs including dUTP – Enables UNG-based carryover contamination prevention
• Internal ROX-based reference dye – Compatible with most cyclers
• 100% DMSO (separate vial) – Optional additive for high GC templates or secondary structures

Product Specifications

Ordering Information

The HOT FIREPol® Probe Universal qPCR Kit provides a robust, flexible, and highly specific solution for probe-based qPCR applications. With its optimized formulation for both AT- and GC-rich templates, internal ROX reference dye, and contamination-prevention system, it is an ideal choice for quantitative gene expression, microbial detection, and viral load studies across a wide range of real-time PCR platforms.