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HOT FIREPol® Blend Master Mix Ready to Load
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Specifications:
| Application | PCR | ||
| Storage Temperature | Room Temperature | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | SolisBiodyne | ||
| Product Grade | Molecular Biology | ||
HOT FIREPol® Blend Master Mix Ready to Load is a high-fidelity, hot-start, and gel-loading-compatible PCR master mix designed for high-demand applications requiring maximum yield, sensitivity, and specificity. This ready-to-use 5× mix combines the power of HOT FIREPol® DNA polymerase with a proofreading enzyme, resulting in up to 5× higher fidelity than standard Taq polymerase.
With its direct gel-loading capability (blue/yellow tracking dyes and loading agent), this mix is ideal for rapid PCR amplification and immediate electrophoretic analysis—eliminating the need for separate loading buffers. It supports amplification of targets up to 5 kb, making it suitable for a wide range of PCR protocols, including blunt-end and T/A cloning.
Key Features and Benefits
- Hot-start enzyme system: reduces non-specific amplification
- High fidelity: Up to 5× more accurate than regular Taq
- Direct-to-gel loading: Blue and yellow dyes + density agent included
- Proofreading capability: Enhances fidelity and fragment length
- Long-range PCR: Amplifies DNA fragments up to 5 kb
- Cloning-compatible: PCR products suitable for both T/A and blunt-end cloning
- Ready-to-use format: Reduces pipetting steps and contamination risk
- Available in multiple MgCl₂ concentrations for protocol flexibility
Applications
- High-fidelity PCR and routine gene amplification
- Cloning (T/A or blunt-end)
- DNA fragment screening by agarose gel electrophoresis
- GC-rich or challenging template amplification
- Ideal for any PCR assay requiring post-PCR gel visualization
Technical Specifications
| Attribute | Description |
|---|---|
| Format | 5× concentrated PCR Master Mix |
| Polymerase | HOT FIREPol® DNA polymerase + proofreading enzyme |
| Hot Start Activation | Yes, 12–15 min initial activation |
| Fidelity | Up to 5× Taq polymerase |
| Direct Gel Loading | Yes (includes loading agent + dyes) |
| Dyes Included | Blue dye (~3.5–4.5 kb), Yellow dye (<35–45 bp) |
| MgCl₂ Concentration (1×) | 1.5 mM standard (also available in 2.0 mM and 2.5 mM) |
| dNTPs | 200 µM each (dATP, dCTP, dGTP, dTTP) |
| BSA | Included to enhance amplification efficiency |
| Amplicon Length Capability | Up to 5 kb |
| Shipping | Ambient or on dry ice depending on distributor protocol |
| Regulatory Status | Research Use Only |
Limitations
- Not suitable for absorbance or fluorescence-based applications (dyes may interfere)
- Not recommended for quantitative PCR or spectrophotometric detection workflows
HOT FIREPol® Blend Master Mix Ready to Load combines robust enzyme performance, hot-start specificity, and direct gel-loading convenience in a single ready-to-use master mix. Ideal for researchers who demand high-fidelity PCR, it offers a powerful solution for fast, accurate, and efficient amplification of DNA in a wide variety of routine and advanced applications.
- MgCl2 final concentration: 1.5 mM 2.0 mM 2.5 mM
- Pack Size: 1 x 1 mL, sufficient for 250 Rxns 5 x 1 mL, sufficient for 1250 Rxns 10 x 1 mL, sufficient for 2500 Rxns 1 x 20 mL, sufficient for 5000 Rxns 0.1 mL, sufficient for 25 Rxns (free sample)