GoTaq® G2 DNA Polymerase

GoTaq® G2 DNA Polymerase is the second generation of GoTaq® products designed to reliably amplify a wide range of PCR templates with improved performance. This enzyme is based on Taq DNA polymerase, featuring 5' → 3' exonuclease activity, and is engineered for better consistency and reliability. With its proprietary formulation, including 50% glycerol and optimized buffers, GoTaq® G2 provides enhanced amplification results, ensuring high yields and consistent performance across various PCR applications.

GoTaq® G2 is available with 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer, both of which contain MgCl₂ for efficient amplification. The green buffer includes dyes that help track migration progress during electrophoresis, while the colorless buffer is ideal for experiments requiring direct fluorescence or absorbance readings without prior DNA purification.

Key Features & Benefits:

1. Enhanced PCR Performance:
   • Improved manufacturing processes and buffer formulations ensure high performance and reliable amplification across a variety of templates, including challenging targets.
2. Reliable Amplification of Multiple Targets:
   • GoTaq® G2 consistently produces high yields across different PCR targets. When tested, GoTaq® G2 outperformed other Taq polymerases (including competitors such as T, Q, L1, L2, and R) in amplifying multiple targets from human DNA.
3. Exonuclease Activity:
   • The enzyme has 5' → 3' exonuclease activity, which aids in the degradation of primer dimers, ensuring efficient amplification and reducing artifacts in PCR.
4. Two Buffer Options:
   • 5X Green GoTaq® Reaction Buffer: Contains two dyes (blue and yellow) that separate during electrophoresis, providing a visual indicator of migration progress. This buffer also increases sample density, allowing for direct loading onto gels without additional loading dye.
   • 5X Colorless GoTaq® Reaction Buffer: Ideal for applications requiring direct fluorescence or absorbance measurements, without the need to purify the amplified DNA.
5. Performance Guarantee:
   • Promega's PCR products come with a performance guarantee. If you are not satisfied with the results, Promega offers a replacement or refund for the product.
6. Versatile Use:
   • GoTaq® G2 DNA Polymerase is suitable for a wide range of molecular biology applications, including routine PCR, cloning, genotyping, quantitative PCR (qPCR), and other amplification needs.

Applications:

1. DNA Amplification:
   • Ideal for routine PCR applications, where efficient amplification of multiple targets is needed.
2. Cloning and Gene Expression:
   • Perfect for use in cloning experiments and gene expression analysis, where high yield and efficient amplification are required.
3. Genotyping:
   • Suitable for genotyping applications such as SNP analysis or mutant identification in genetic studies.
4. Quantitative PCR (qPCR):
   • Compatible with quantitative PCR applications where accurate DNA amplification is essential for quantification.

Specifications:

Contents:

• GoTaq® G2 DNA Polymerase (20–25 U/μL)
• 5X Green GoTaq® Reaction Buffer
• 5X Colorless GoTaq® Reaction Buffer

Storage:

• Store GoTaq® G2 DNA Polymerase at -20°C. Keep the enzyme and buffers tightly sealed to prevent degradation.

GoTaq® G2 DNA Polymerase is an ideal choice for efficient and reliable PCR across a wide range of applications. With its improved performance, high transformation efficiency, and two buffer options, it provides consistent, high-quality results, whether you're working on routine DNA amplification, cloning, or gene expression analysis. It is perfect for labs requiring a high-performance polymerase for molecular biology research.