Gibco™ VP-SFM AGT™ Medium, Powder

Gibco™ VP-SFM AGT™ Medium is a powdered, granular, serum-free, ultra-low protein medium (5 µg/mL) designed for the growth of VERO cells for virus production. It is also compatible with COS-7, MDCK, BHK-21, and HEp2 cells and supports recombinant protein production. This advanced granular technology (AGT™) format ensures convenience, consistency, and reduced contamination risk in cell culture workflows.

Key Features:

1. Granular Format (AGT™):
   • Easy-to-use powdered medium reduces dust, enhances handling, and minimizes contamination risks.
2. Serum-Free and Animal-Origin Free:
   • Eliminates variability and contamination risks associated with serum and animal-derived components.
3. Ultra-Low Protein Content:
   • Contains 5 µg/mL of protein, reducing interference in downstream applications.
4. Broad Cell Line Compatibility:
   • Optimized for VERO cells and suitable for other cell lines, including COS-7, MDCK, BHK-21, and HEp2.
5. Customizable with Additives:
   • Includes HEPES, sodium pyruvate, phenol red, and sodium bicarbonate.
   • Formulated without L-glutamine, allowing users to add their preferred supplement.
6. Certified Quality:
   • Manufactured under stringent conditions to ensure consistency and compliance with research and manufacturing requirements.

Specifications:

Applications:

1. Virus Production:
   • Specially designed for VERO cells to support large-scale virus production for vaccine research.
2. Recombinant Protein Production:
   • Compatible with mammalian cell lines like COS-7 and BHK-21 for producing therapeutic or research-grade proteins.
3. General Mammalian Cell Culture:
   • Suitable for MDCK, HEp2, and other adherent or suspension cell lines.
4. Large-Scale Biomanufacturing:
   • Powdered format enables scalable production in research and industrial settings.
5. Customizable Protocols:
   • Allows users to tailor the medium by adding L-glutamine or other supplements to meet specific research needs.

Advantages:

1. Convenience and Consistency:
   • Granular format reduces preparation errors, ensuring reproducibility and ease of use.
2. Regulatory Compliance:
   • Animal-origin free and serum-free formulation meets stringent quality requirements for advanced research and manufacturing.
3. Reduced Contamination Risk:
   • Low endotoxin levels and defined composition minimize variability and contamination.
4. Customizable Flexibility:
   • Compatible with a wide range of additives to suit various applications.
5. Eco-Friendly:
   • Powdered format reduces packaging waste and storage footprint.

Preparation Instructions:

1. Rehydrate Medium:
   • Dissolve the required amount of VP-SFM AGT™ powder in distilled water.
2. Add Supplements:
   • Add L-glutamine or GlutaMAX™ to the final working concentration.
3. Sterilize:
   • Filter-sterilize the prepared medium using a 0.22 µm filter.
4. Adjust pH:
   • Ensure the medium pH is between 6.9 and 7.4 before use.
5. Store and Use:
   • Store reconstituted medium at 2–8°C and protect from light.

Ordering Information:

Storage and Handling:

• Storage Conditions: Store at 2–8°C, protected from light.
• Shelf Life: Refer to product label for expiration date.

Related Products:

• Gibco™ GlutaMAX™ Supplement: For stable L-glutamine supplementation.
• Gibco™ DPBS Without Calcium and Magnesium: For cell washing and preparation.
• Gibco™ VP-SFM Liquid Medium (1X): Ready-to-use liquid alternative to AGT™ powder.

Regulatory and Manufacturing Notes:

• For Research Use Only: Not intended for diagnostic or therapeutic applications.
• Quality Manufacturing: Manufactured to meet stringent standards for research and manufacturing workflows.

The Gibco™ VP-SFM AGT™ Medium, Powder offers unparalleled convenience, consistency, and compatibility for virus production, recombinant protein research, and general mammalian cell culture. Its granular format, high-quality formulation, and regulatory readiness make it a reliable choice for advanced applications.