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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Crystals |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ OptiPRO™ Serum-Free Medium (SFM) is a chemically defined, serum-free, animal origin–free, low-protein culture medium designed for the growth of kidney-derived cell lines, including MDBK, MDCK, PK-15, and VERO, commonly used in virus production and recombinant protein expression. In addition, OptiPRO™ SFM supports the growth of other mammalian cell lines such as HeLa, BHK-21, and COS-7, offering a versatile solution for multiple culture needs.
With a protein concentration of <10 µg/mL, OptiPRO™ SFM simplifies downstream purification of recombinant proteins. Manufactured under cGMP conditions in a facility compliant with ISO 13485 and FDA-registered, this medium provides consistent quality and performance for research and further manufacturing applications.
Key Features
- Serum-Free and Animal Origin-Free – Reduces variability and eliminates animal-derived components.
- Low Protein Content (<10 µg/mL) – Facilitates efficient purification of recombinant proteins.
- Versatile Cell Line Compatibility – Optimized for MDBK, MDCK, PK-15, and VERO; validated for HeLa, BHK-21, COS-7, and more.
- Direct Adaptation – Enables smooth transition of many cell lines to serum-free conditions.
- Manufactured to cGMP Standards – Produced in an FDA-registered, ISO 13485–certified facility.
Applications
- Growth and maintenance of kidney-derived and other mammalian cell lines.
- Virus production (e.g., for vaccine development).
- Recombinant protein production requiring low-protein medium for downstream processing.
- Research and further manufacturing workflows requiring defined, serum-free formulations.
Technical Specifications
| Parameter | Details |
|---|---|
| Catalog Numbers | 12309019 (1000 mL), 12309050 (100 mL) |
| Format | Liquid medium, 1X |
| Protein Concentration | <10 µg/mL |
| Cell Line Compatibility | MDBK, MDCK, PK-15, VERO, HeLa, BHK-21, COS-7 |
| Serum Level | Serum-free |
| Animal Origin | Animal origin–free |
| With Additives | Phenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine |
| Without Additives | No glutamine |
| Culture Type | Adherent cell culture |
| Endotoxin Level | Low |
| Storage Conditions | 2–8 °C, protect from light |
| Shelf Life | 24 months from date of manufacture |
| Shipping Conditions | Ambient |
| Unit Sizes | 100 mL, 1000 mL |
Unique Advantages
- Provides a defined, consistent formulation for reproducible cell culture.
- Simplifies downstream viral and protein purification workflows due to low protein content.
- Eliminates risks of contamination from serum or animal-derived components.
- A single medium solution for multiple mammalian cell lines, increasing convenience and cost-effectiveness.
The Gibco™ OptiPRO™ SFM is a versatile, serum-free, animal origin–free medium designed to support a broad range of mammalian cell lines, particularly kidney-derived lines widely used in virus and recombinant protein production. With its low-protein formulation, cGMP manufacturing, and ISO 13485 certification, it ensures consistency, reproducibility, and efficiency across research and manufacturing workflows.
- Pack Size: 100 mL 1000 mL